β-七叶皂苷早期应用对大鼠脊髓损伤的保护作用  

Protective effects of β-aescin on spinal cord injury in the rat

作　　者：程鹏[1] 冯斌[2] 鞠躬[1] 

机构地区：[1]第四军医大学全军神经科学研究所,西安710032 [2]第四军医大学药学院药理学教研室,西安710032

出　　处：《神经解剖学杂志》2011年第2期119-123,共5页Chinese Journal of Neuroanatomy

基　　金：国家重大新药创制大平台项目(2009ZX09301-009-PT02);军队"十一五"专项(08Z028)

摘　　要：目的:观察β-七叶皂苷(β-aescin)对大鼠脊髓损伤后继发性损害的保护作用。方法:本实验采用NYUⅡ型脊髓撞击伤仪制成中度脊髓损伤模型。术后30 min,所有大鼠分别经腹腔给予β-七叶皂苷(1.0 mg/kg)或盐水治疗,3次/d,连给3 d。术后,于各时间点采用BBB评分和足迹实验对大鼠双后肢运动功能进行评估;术后24 h,对损伤部位脊髓组织丙二醛(MDA)含量及髓过氧化物酶(MPO)活性进行检测;术后14 d行免疫组织化学检查,利用胶质原纤维酸性蛋白(GFAP)观察损伤星形胶质瘢痕及其包围的坏死空洞,利用抗CD68/ED1观察活性小胶质细胞和巨噬细胞。结果:β-七叶皂苷组运动功能明显改善(P<0.05);伤后24 h,β-七叶皂苷组大鼠脊髓组织中MDA水平降低67%(P<0.01),MPO活性降低50%(P<0.01);伤后14 d,β-七叶皂苷组空洞面积减小29.3%(P<0.01),ED1染色阳性面积减少32.3%(P<0.01)。结论:β-七叶皂苷治疗可以减轻脊髓损伤后自由基的氧化损伤作用,减小空洞面积,减少炎症细胞的活化和浸润,促进脊髓损伤后的修复。Objective：To investigate the protective effects of β-aescin on acute traumatic spinal cord injury（SCI） in rats.Methods：We examined the hypothesis that β-aescin has protective effects in a rat model of moderate spinal cord contusion induced by NYU Impactor（Type Ⅱ）.Thirty min after injury,all rats were treated with β-aescin（1.0 mg/kg body weight i.p.） or normal saline,and received additional doses 3 times a day for 3 consecutive days.Locomotor deficits induced by SCI were assessed using the BBB score and footprint analysis.Biochemical assays were executed at 24 h postinjury to detect the level of malondialdehyde（MDA） and the activity of myeloperoxydase（MPO）.14 days after injury,histological tests were performed to analyze the lesion size using GFAP,and activated microglia and recruited macrophages using anti-CD68/ED1.Results：Our data indicated that β-aescin treatment significantly accelerated locomotor function recovery（P〈0.05）,reduced MDA level by 67%（P〈0.01） and MPO activity by 50%（P〈0.01）,decreased lesion cavitation by 29.3%（P〈0.01） and ED1 positive area by 32.3%（P〈0.01）.Conclusion：The present study showed that β-aescin treatment strikingly reduced lipid peroxydation（LPO）,suppressed microglia activation and hematogenous leukocyte infiltration,and facilitated reparation after SCI.

关 键 词：β--七叶皂苷 脊髓损伤 脂质过氧化 炎症反应 大鼠 

分 类 号：R285.5[医药卫生—中药学]