内蒙古呼伦贝尔草原土壤氨氧化细菌多样性及群落结构  

Diversity and community structure of soil ammonia-oxidizing bacteria in Hulunbeier Grassland,Inner Mongolia

作　　者：文都日乐[1,2] 李刚[1] 杨殿林[1] 张静妮[1] 易津[2] 

机构地区：[1]农业部环境保护科研监测所,天津300191 [2]内蒙古农业大学农学院,呼和浩特010018

出　　处：《应用生态学报》2011年第4期929-935,共7页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(31000242;30770367);中国农业科学院院长基金项目资助

摘　　要：采用聚合酶链式反应-变性梯度凝胶电泳技术及扩增产物序列分析方法,研究了呼伦贝尔5种草地类型(线叶菊草原、贝加尔针茅草原、羊草草原、大针茅草原、克氏针茅草原)土壤氨氧化细菌多样性及群落结构特征.研究表明:不同草地类型间土壤氨氧化细菌群落结构组成差异显著,相似性均低于50%.线叶菊草原土壤氨氧化细菌群落多样性最高,其次是贝加尔针茅草原、羊草草原和克氏针茅草原,大针茅草原最低.5种草地类型土壤氨氧化细菌均以Nitrosospira cluster3为优势种群,此外还发现有Nitrosospira cluster1、2、4和Nitrosomonas.线叶菊草原土壤氨氧化细菌群落组成较其他草地类型复杂,而羊草草原和大针茅草原群落组成较简单.经相关性分析,土壤含水量、土壤全氮、有机碳、土壤C/N与土壤氨氧化细菌群落多样性显著正相关(P<0.05).By the methods of polymerase chain reaction-denaturing gradient gel electrophoresis and sequence analysis,a comparative study was conducted on the diversity and community structure of soil ammonia-oxidizing bacteria in the Filifolium sibiricum steppe,Stipa baicalensis steppe,Leymus chinensis steppe,Stipa grandis steppe,and Stipa kryrowi steppe in Hulunbeier Grassland,Inner Mongolia.A significant difference was observed in the community structure of soil ammonia-oxidizing bacteria among the five steppes,with the similarity lower than 50%.The diversity of soil ammonia-oxidizing bacteria was the highest in F.sibiricum steppe,followed by in S.baicalensis steppe,L.chinensis steppe,S.kryrowi steppe,and S.grandis steppe.In the five steppes,Nitrosospira cluster 3 was the dominant group,and the Nitrosospira cluster 1,2,and 4 as well as Nitrosomonas were also found.The community structure of soil ammonia oxidizing bacteria in F.sibiricum steppe was most complex,while that in L.chinensis steppe and S.grandis steppe was relatively simple.Correlation analysis indicated that there existed significant positive correlations between the diversity of soil ammonia-oxidizing bacteria and the soil moisture,total nitrogen,total organic carbon,and C/N ratio（P0.05）.

关 键 词：呼伦贝尔草原 氨氧化细菌 多样性 系统发育分析 

分 类 号：S812.2[农业科学—草业科学]