不同浓度Ni、Cu处理对骆驼蓬光合作用和叶绿素荧光特性的影响  

Influence of different concentration Ni and Cu on the photosynthesis and chlorophyll fluorescence characteristics of Peganum harmala

作　　者：鲁艳[1,2] 李新荣[3] 何明珠[3] 苏延桂[1] 曾凡江[1,2] 

机构地区：[1]中国科学院新疆生态与地理研究所,乌鲁木齐830011 [2]新疆策勒荒漠草地生态系统国家野外科学观测研究站,新疆策勒848300 [3]中国科学院寒区旱区环境与工程研究所沙坡头沙漠研究试验站,兰州730000

出　　处：《应用生态学报》2011年第4期936-942,共7页Chinese Journal of Applied Ecology

基　　金：新疆维吾尔自治区科技重大专项(200733144-2);国家杰出青年科学基金项目(40825001);国家科技支撑计划项目(2009BAC54B01)资助

摘　　要：以骆驼蓬幼苗为材料,采用盆栽试验研究不同浓度(0、50、100、200、400 mg.kg-1)Ni、Cu处理对骆驼蓬叶片光合作用、叶绿素荧光特性及生长状况的影响.结果表明:随着Ni浓度的增加,骆驼蓬幼苗叶片的光合色素含量、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、PSⅡ最大光化学效率(Fv/Fm)、PSⅡ电子传递量子产率(ΦPSⅡ)、光化学猝灭系数(qP)及各项生长指标均呈显著下降趋势,而细胞间隙CO2浓度(Ci)和非光化学猝灭系数(qN)呈显著增加趋势,其中Pn的下降主要是由非气孔限制所致;骆驼蓬幼苗叶片的光合色素含量、Pn、Gs、Tr、Ci、Fv/Fm、ΦPSⅡ、qP及各项生长指标均在50 mg.kg-1 Cu处理时达到峰值,叶绿素a和b、Pn、Gs、Tr、Ci、Fv/Fm及各项生长指标值在100 mg.kg-1 Cu处理时仍微高于对照,而后随Cu浓度的增加,光合色素含量、Pn、Gs、Tr、Ci、Fv/Fm、ΦPSⅡ、qP及各项生长指标均呈下降趋势,qN呈增加趋势,其中Pn的下降主要是由气孔限制所致.A pot experiment was conducted to study the influence of different concentration（0,50,100,200,and 400 mg·kg-1） Ni and Cu on the growth,photosynthesis,and chlorophyll fluorescence characteristics of Peganum harmala seedlings.With increasing concentration Ni in the medium,the seedlings growth parameters,photosynthetic pigment content,net photosynthetic rate（Pn）,stomatal conductance（Gs）,transpiration rate（Tr）,maximal photochemical efficiency of PSⅡ（Fv/Fm）,quantum efficiency of electric transport of PSⅡ（ΦPSⅡ）,and coefficient of photochemical quenching（qP） of P.harmala decreased significantly,while the intercellular CO2 concentration（Ci） and the coefficient of non-photochemical quenching（qN） were in adverse.The decrease of Pn under Ni stress was mainly caused by non-stomatal limitation.At 50 mg·kg-1 Cu,the growth parameters,photosynthetic pigment content,Pn,Gs,Tr,Ci,Fv/Fm,ΦPSⅡ,and qP reached their peak values;at 100 mg·kg-1 Cu,the growth parameters,chlorophyll a and b contents,Pn,Gs,Tr,Ci,and Fv/Fm were still slightly higher than the control;while with the further increasing Cu concentration in the medium,all the test indices except qN tended to decrease.The decrease of Pn under Cu stress was mainly caused by stomatal limitation.

关 键 词：光合作用 叶绿素荧光 骆驼蓬 镍 铜 

分 类 号：S793.9[农业科学—林木遗传育种]