血流导向支架置入后内皮祖细胞对兔动脉瘤的促愈合作用  

Role of endothelial progenitor cells in healing aneurysms with flow diverting stents

作　　者：方兴根[1] 赵瑞[1] 王奎重[1] 李振[1] 黄清海[1] 许奕[1] 洪波[1] 刘建民[1] 

机构地区：[1]第二军医大学附属长海医院神经外科,上海200433

出　　处：《中国脑血管病杂志》2011年第2期87-90,共4页Chinese Journal of Cerebrovascular Diseases

基　　金：国家自然科学基金资助项目(30901556;81000495)

摘　　要：目的探讨内皮祖细胞(EPCs)能否参与血流导向支架治疗动脉瘤的早期愈合过程。方法选择清洁级大白兔5只,采用胰弹性酶制备右侧颈动脉动脉瘤模型。自体培养EPCs后,用Hoechst 33342干和羧基荧光素二醋酸盐琥珀酰亚胺酯(CFSE)进行双标记。利用血流导向支架治疗兔的动脉瘤,将标记的EPCs经静脉自体回输,2周后利用细胞示踪技术观察EPCs参与动脉瘤内膜的修复情况,结合扫描电镜、病理、免疫组化等方法分析EPCs参与内膜修复的过程。结果①单纯血流导向支架治疗后2周复查,动脉瘤完全不显影(5/5),动脉瘤完全闭塞,瘤颈口覆盖新生内膜;②在2只实验动物(2/5)的动脉瘤切片中发现H1oechst 33342和CFSE双标记的阳性细胞,阳性细胞位于新生的内膜内;③扫描电镜及免疫组化结果提示,内膜面仅有少量内皮细胞修复内膜。结论 EPCs参与了动脉瘤的早期内膜愈合过程。Objective To investigate whether endothelial progenitor cells （EPCs） involved in the early healing process of aneurysms with flow diverting stents and the mechanisms. Methods Five clean rabbits were chosen to prepare the animal model of aneurysm on the fight carotid artery. After EPCs were cultured autologously, they were double labeled by Hoechst 33342 and carboxyflnorescein diacetate succinimidylester （CFSE）. The aneurysnls of rabbits were treated using flow diverting stents. The labeled EPCs were retransfused autologously via veins. EPCs involving in the intimal repair of aneurysms were observed with Cell tracking technology after 2 weeks. In combination of scanning electron microscopy, pathology and immunohistochemistry, EPCs involving in the process of intimal repair were analyzed. Results ①Two weeks after simple flow diverting stents treatment, the rabbits were reexamined. Their aneurysms did not visualized com- pletely （5/5）. The aneurysms were obliferated completely, and the aneurysm necks were covered with neointima. ②Hoechst33342 and CFSE double-labeled positive cells were found in anenrysm sections. In the aneu-rysm sections of 2 experimental animals （2/5）, the positive cells were in the neointima. ③Scanning electron microscopy and immunohistoehemistry results suggested that only a few amount of endothelial ceils repaired the intimal surface. Conclusion EPCs involved in the process of early intimal healing of aneurysms.

关 键 词：颅内动脉瘤 内皮细胞 支架 兔 

分 类 号：R543.5[医药卫生—心血管疾病]