人脂肪干细胞对兔任意型皮瓣成活的影响  

The effect of adipose-derived stem cells on viability of random pattern skin flap in rabbits

作　　者：李光早[1] 孙庆章[1] 熊竹友[1] 黄鹤[1] 徐静[1] 

机构地区：[1]蚌埠医学院第一附属医院整形外科,组织移植安徽省重点实验室,233004

出　　处：《中华整形外科杂志》2011年第2期119-123,共5页Chinese Journal of Plastic Surgery

摘　　要：目的 探索人脂肪干细胞（adipose-derived stem cells,ADSCs）对兔任意型皮瓣成活能力的影响.方法 获得健康成人的脂肪,经体外分离、培养及传代至第5代,进行流式细胞术检测和三系分化能力的鉴定.于大白兔背部对称地设计2个蒂部在头侧的任意型皮瓣（长6 cm、宽2 cm）,左、右侧分别为对照组和实验组.应用DiO荧光标记ADSCs,实验组（n=10）分5点注射2×106悬浮于0.5 ml无血清的DMEM培养基中的ADSCs,对照组（n=10）同样分5点注入无血清的DMEM0.5 ml,于术后7 d分别测量皮瓣成活面积;随机作冰冻切片,在激光扫描共聚焦显微镜下观察切片荧光显影情况;随机作常规切片HE染色,光镜下观察皮瓣组织毛细血管分布密度.统计学分析ADSCs在促进任意型皮瓣成活过程中的作用.结果 与对照组相比,实验组皮瓣成活率明显提高（P〈0.01）,组织学检测证明实验组毛细血管增生明显增多.结论 人ADSCs具有较好的免疫相容性,局部应用可明显提高皮瓣的成活率.Objective To explore the effect of the adipose derived stem cells （ADSCs）on the survival of random pattern skin flap in rabbits. Methods ADSCs were isolated from fresh human fat and expanded in vitro for five passages. Then the characterization of ADSCs were determined by their CD marker profile and their ability to differentiate into osteogenic, adipogenic and chondrogenic lineages. On the back of the rabbits, two symmetric cephalic-based random pattern skin flaps were designed （6 cm × 2cm）. The right flaps were used as experimental groups with the contra-side flaps as control group. Human ADSCs were pre-labeled before seeding with fluorescent 3, 30-dioctadecyloxacarbocyanine perchlorate （DiO） dye. The experimental flaps evenly received 5-point injection of 2 × 106Dio-labeled ADSCs resuspended in 0.5 ml of serum-free DMEM, while only 0. 5 ml medium was injected into the control flaps.Seven days later, the survival rate of flaps was evaluated. The flaps underwent frozen section and were observed under the laser scanning confocal microscope to detect the fluorescence imaging. Flaps also underwent HE staining and were observed under light microscope to detect the vascular density. Results Compared with control group, there was a significant increase of flap survival rate in the experimental group（P 〈0.01）. Histological analysis also demonstrated a statistically significant increase in capillary density in the experimental group. Conclusions It suggests that ADSCs have a better immune compatibility and potential for enhancing the blood supply of random pattern skin flaps.

关 键 词：脂肪干细胞 外科皮瓣 组织工程 

分 类 号：R622[医药卫生—整形外科]