广东籍汉族妇女N5,10-MTHFR基因和eNOS基因多态性与子痫前期和子痫相关性的研究  

Study on the relationship between N5,10-methylenetetrahydrofolate reductase gene and endothelial nitric oxide synthase gene polymorphisms and preeclampsia and eclampsia in the Han nationality women of Guangdong

作　　者：苏念军[1] 李冰[1] 冯健怀[1] 于滨[1] 

机构地区：[1]广州医学院第三附属医院妇产科,广州510150

出　　处：《现代妇产科进展》2011年第3期199-202,共4页Progress in Obstetrics and Gynecology

基　　金：广东省自然科学基金资助课题(No:2006B35901018)

摘　　要：目的:探讨N5,10-亚甲基四氢叶酸还原酶(MTHFR)基因及内皮型一氧化氮合酶(eNOS)基因多态性与广东籍汉族妇女子痫前期和子痫发病的关系。方法:567例广东籍汉族妇女中54例诊断为子痫前期或子痫,513例为正常妊娠(对照组)。应用PCR-RFLP方法,检测567例早孕期妇女外周血MTHFR基因C677T突变和eNOS基因G894T突变,计算各基因型的相对风险率。结果:子痫前期和子痫组MTHFR C/C、C/T及T/T基因型频率分别为59.2%、20.4%及20.4%,其中T/T基因型频率显著高于对照组(5.5%)(P<0.001),并且T等位基因频率为30.6%,显著高于对照组(20.5%)(P<0.05),T/T基因型在子痫前期或子痫发病中的相对风险率为4.431。子痫前期和子痫组的eNOS基因频率与对照组无显著差异。结论:广东籍汉族妇女MTHFR基因C677T多态性可能与子痫前期或子痫发病的易感性相关,eNOS基因G894T多态性与子痫前期或子痫发病的易感性无关。Objective：To investigate a potential association of the gene polymorphisms of N5,10-methylenetetrahydrofolate reductase（MTHFR）gene and endothelial nitric oxide synthase（eNOS）gene with preeclampsia and eclampsia in the Han nationality women of Guangdong,China.Methods：The MTHFR C677T polymorphisms and eNOS G894T polymorphisms were determined by PCR-RFLP in 54 pregnancy induced hypertensions patients and 513 normal early pregnancy women（control group）.Odds ratios（OR） and 95% CI were obtained to evaluate the association between the polymorphisms and preeclampsia and eclampsia.Results：The frequencies of MTHFR C/C,C/T and T/T genotypes in preeclampsia and eclampsia group were 59.2%,20.4% and 20.4%,respectively.The frequencies of T/T genotype in preeclampsia and eclampsia group were significantly higher than those in control group（5.5%,P0.001）,moreover,the frequencies of T allele was 30.6%,which was significantly higher than those in control group（20.5%,P0.05）.Furthermore,the risk rate of T/T genotype was 4.431.There were no difference in eNOS G894T polymorphisms in preeclampsia and eclampsia group and control group.Conclusion：MTHFR C677T polymorphisms may be the high risk factors of pregnancy induced hypertensions in the Han nationality women in Guangdong,China.On the other hand,eNOS G894T polymorphisms may not be the high risk factors of pregnancy induced hypertensions in the same population.

关 键 词：子痫前期 子痫 N5 10-亚甲基四氢叶酸还原酶 内皮型一氧化氮合酶 基因多态性 

分 类 号：R711.6[医药卫生—妇产科学]