母儿中HLA-G 14bp插入/缺失多态性在早发型、晚发型重度子痫前期和正常妊娠中分布的差异  

The distribution difference of HLA-G 14bp insertion/deletion polymorphism in maternal-child between early(late)onset of severe pre-eclampsia and normal pregnancies

作　　者：李红[1] 张琳琳[1] 贾莉婷[1] 石瑛[1] 张楠[1] 张展[1] 

机构地区：[1]郑州大学第三附属医院,郑州450052

出　　处：《现代妇产科进展》2011年第3期203-206,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金项目(No:30872316)

摘　　要：目的:探讨早发型、晚发型重度子痫前期和正常妊娠中母儿HLA-G 14bp插入/缺失多态性分布的差异。方法:(1)分析早发型重度子痫前期31例,晚发型重度子痫前期25例,正常妊娠组30例;(2)采用聚合酶链反应技术(PCR),对3组母儿进行HLA-G基因第8外显子14bp插入/缺失多态性的等位基因分型,分别比较3组母亲之间和3组新生儿之间等位基因及基因型的频率分布,通过母/儿基因型配伍,比较3组间基因型配伍频率分布的差异。结果:(1)早发型重度子痫前期组母/儿基因型配伍为母缺失纯合子(-/-14bp)/儿杂合子(+/-14bp)出现的几率要高于晚发型组(χ2=5.034,P=0.025),其和正常妊娠组相比有升高的趋势(χ2=3.685,P=0.055);早发型重度子痫前期组母/儿基因型配伍为母杂合子(+/-14bp)/儿缺失纯合子(-/-14bp)出现的几率要低于正常妊娠组(χ2=3.985,P=0.046);(2)正常妊娠新生儿组HLA-G 14bp缺失多态性等位基因频率分布高于早发型组(χ2=6.270,P=0.012),缺失纯合子(-/-14bp)基因型频率分布高于早发型组(χ2=6.139,P=0.013)。结论:(1)早发型重度子痫组母/儿HLA-G 14bp插入/缺失多态性基因型配伍的不同可能引起母儿之间免疫耐受异常,导致了早发型重度子痫前期的发生;(2)胎儿HLA-G 14bp缺失多态性可能降低了早发型重度子痫前期的发生。Objective：To investigate the distribution difference of HLA-G 14bp insertion/deletion polymorphism in maternal-child between early（late） onset of severe pre-eclampsia（PE） and normal pregnancies.Methods：Analysis 31 cases of early onset severe PE,25 cases of late onset severe PE and 30 cases of normal pregnancies,HLA-G Exon 8 genotyping was performed by polymerase chain reaction（PCR） in three groups including women and their neonates in which Chinese Han population.Respectively compare the distributions of the allele frequencies and genotype frequencies of the three groups,combine maternal and their neonates to analyze the differences of the genotype special bindings.Results：（1） The frequency distribution of maternal（HLA-G-/-14bp）/fetus（HLA-G＋/-14bp） genotype binding in the early onset group was significantly higher than late onset group（χ2=5.034,P=0.025）,and with differences in the trend compare the normal pregnancies（χ2=3.685,P=0.055）.The frequency distribution of maternal（HLA-G＋/-14bp）/fetus（HLA-G-/-14bp） genotype binding in the early onset group was significantly lower than normal pregnancies group（χ2=3.985,P=0.046）.（2）Neonates within normal group of 14bp deletion polymorphism allele frequency and（-14bp/-14bp） genotype frequency was significantly higher than the early onset group,the difference statistically significance was（χ2=6.270,P=0.012）and（χ2=6.139,P=0.013） respectively.Conclusion：（1）The different genotypes compatibility of HLA-G 14bp insertion/deletion polymorphism in maternal-child of Early onset severe PE may cause the immune tolerance abnormalities,led to the early onset severe PE.（2） The 14bp deletion polymorphism of neonates may reduce the occurrence of early onset severe PE.

关 键 词：人类白细胞抗原 早发型、晚发型重度子痫前期 HLA-G 14bp多态性 

分 类 号：R714[医药卫生—妇产科学]