利用人工microRNA实现基因沉默  

Specific Gene Silencing by Artificial MicroRNA

作　　者：陈起振[1] 张志仙[1] 曹家树[1] 

机构地区：[1]浙江大学蔬菜研究所,杭州310029

出　　处：《中国细胞生物学学报》2011年第4期433-438,共6页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.30871715)资助项目~~

摘　　要：MicroRNA是一组长度约为21 nt的非编码蛋白质的短序列RNA,能通过碱基互补配对的方式指导降解靶基因mRNA或抑制靶基因的翻译。MicroRNA的主要功能是调控基因的表达,在生物体的生长、发育及疾病发生中扮演着重要的角色。本文介绍了利用microRNA实现基因沉默的作用原理,人工合成microRNA,构建转基因载体,实现对目的基因的沉默及这种工具在生命科学领域的应用前景。MicroRNAs are post-transcriptional regulators, composed by approximately 21 ribonucleic acid （RNA） molecules. By binding to complementary sequences of target genes, they can cause degradation of the mRNAs of target genes or inhibit their translation. MicroRNAs have been implicated in processes and pathways such as development, cell proliferation, apoptosis, metabolism and morphogenesis, and in diseases including cancer. This paper aimed to introduce achieving silencing of target gene by using artificial microRNAs, and applications of this method in life science.

关 键 词：人工microRNA RNAI 基因沉默 

分 类 号：Q75[生物学—分子生物学]