眼部蠕形螨寄居患者结膜上皮ICAM-1和HLA-DR的表达和意义  

Expression of ICAM-1 and HLA-DR in conjunctival epithelium of patients with ocular Demodex

作　　者：范春梅[1] 高莹莹[2] 许锻炼[2] 戴炳发[2] 李静[2] 

机构地区：[1]福建医科大学附属第二医院检验科,福建泉州362000 [2]福建医科大学附属第二医院眼科教研室,福建泉州362000

出　　处：《细胞与分子免疫学杂志》2011年第4期450-451,共2页Chinese Journal of Cellular and Molecular Immunology

基　　金：福建省自然科学基金资助项目(2006J0337);泉州市优秀人才培养专项经费资助(2008年)

摘　　要：目的:探讨眼部蠕形螨寄居患者结膜上皮ICAM-1和HLA-DR的表达和意义。方法:选择痤疮、脂溢性皮炎以及类固醇皮炎眼部蠕形螨检查为阳性的患者共58例为蠕形螨寄居组,另选50例无面部疾病眼部蠕形螨检查阴性的健康成人,作为正常对照组。用流式细胞术联合印迹细胞学检测细胞表面分子的方法检测结膜上皮细胞ICAM-1和HLA-DR的表达阳性率及荧光强度。结果:与正常对照组相比,蠕形螨寄居组患者结膜上皮细胞中ICAM-1和HLA-DR的阳性率和荧光强度均明显提高,差异具有统计学意义(P<0.05)。结论:眼部蠕形螨寄居患者结膜上皮中ICAM-1和HLA-DR的表达增强。AIM：Expression of ICAM-1 and HLA-DR in conjunctival epithelium of patients with ocular Demodex was investigation.METHODS： Acne,seborrheic dermatitis and facial steroid dermatitis,skin diseases,and conduct inspections of Demodex total of 58 cases of patients with Demodex stranger group,the other selected 50 patients with non-facial disease in healthy adults as normal control group without Demodex.Flow cytometry combined with blot-cytology was used to detect the expression of HLA-DR.RESULTS： Compared with the normal control group,Demodex sojourn the positive rate and fluorescence intensity in conjunctival epithelial cells of patients with Demodex of ICAM-1 and HLA-DR were significantly increased（P0.05）.DISCUSSION： ICAM-1 and HLA-DR expression is increased in the eye inflammation with Demodex.

关 键 词：蠕形螨 结膜上皮 ICAM-1 HLA-DR 

分 类 号：R392.11[医药卫生—免疫学]