红壤中微生物群落对半导体矿物日光催化作用的响应  

Response of Microbial Community to Sunlight Catalysis of Semiconductor Minerals in Red Soil

作　　者：曾翠平[1] 鲁安怀[1] 李艳[1] 吴婧[1] 王鑫[1] 丁瑞[1] 颜云花[1] 

机构地区：[1]造山带与地壳演化教育部重点实验室北京大学地球与空间科学学院,北京100871

出　　处：《高校地质学报》2011年第1期101-106,共6页Geological Journal of China Universities

基　　金：国家重点基础研究发展计划资助项目(973项目)(2007CB825602)

摘　　要：本研究选取天然红壤作为研究对象,分析其中的含铁半导体矿物光催化作用对本源微生物群落结构的影响。采用聚合酶链式反应/变性梯度凝胶电泳(PCR-DGGE),研究了不同原始光照条件的红壤中微生物群落在外源电子作用下群落结构的改变。DGGE图谱的主成分分析表明,两个不同原始光照环境的土壤样品的微生物群落结构由于原始环境的差异而不同:原始环境为强光照的样品中微生物群落结构受外源电子影响较小;原始环境为弱光照的样品中微生物群落结构受外源电子影响较大。这一群落结构改变的差异可能由于原始环境为强光照时半导体矿物光催化作用产生光生电子传递到环境中持续影响周围的微生物群落,而原始弱光照环境中则缺少光生电子的作用。The natural red soil was used in this study as a research object, and the influence of semiconductor photocatalysis of iron-bearing minerals on microbial community was analyzed. Using the polymerase chain reaction/degeneration gradient gel electrophoresis （PCR-DGGE）, the microbial community from the original red soil samples under different light conditions was investigated after being disposed with exogenous electrons supplement. Principal component analysis （PCA） of DGGE banding patterns indicated that the difference of the microbial community structure in two soil samples was due to different original light environments. In the red soil with strong light, no significant change of the microorganism community structure was observed by processing with exogenous electrons; while in the original environment with weak light, the structure of microbial community was significantly affected by the exogenous electrons. The different response of microbial community structure to the exogenous electrons may be attributed to the photoinduced electrons generated by the semiconducting mineral photocatalysis in the primitive strong irradiation environment, which continued to affect the surrounding microbial communities; while photoinduced electrons were inadequate in the weak irradiation environment.

关 键 词：红壤 微生物群落 PCR-DGGE 外源电子 半导体光催化 

分 类 号：S275.6[农业科学—农业水土工程] S562[农业科学—农业工程]