碳酸钙颗粒的细菌诱导形成  

The Formation of Calcium Carbonate Particles Induced by Bacteria

作　　者：李辉[1] 连宾[2] 龚国洪[3] 杜开和[1] 

机构地区：[1]江苏省功能微生物与功能基因组学重点实验室江苏省微生物工程技术研究中心南京师范大学生命科学学院,南京210046 [2]中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550002 [3]中国科学院地球化学研究所矿床地球化学国家重点实验室,贵阳550002

出　　处：《高校地质学报》2011年第1期112-117,共6页Geological Journal of China Universities

基　　金：国家自然科学基金委创优群体项目(40721002);国家自然科学基金项目(40773069)联合资助

摘　　要：为研究胶质芽孢杆菌(Bacillus mucilaginosus)在特定条件下能否诱导合成碳酸钙,作者采用胶质芽孢杆菌两种常用基本培养基(有氮培养基和无氮培养基),以磷灰石矿物为钙源,进行了利用胶质芽孢杆菌促进碳酸钙形成的实验,借助扫描电镜(SEM)、能谱定量分析(EDS)和X-射线衍射(XRD)等手段观察分析形成碳酸钙的晶体结构和化学组成。结果表明,胶质芽孢杆菌能促进碳酸钙晶体的形成,在有氮加磷矿粉的细菌培养液中形成的碳酸钙多于无氮加磷矿粉的细菌培养液,在有氮加磷矿粉的细菌培养液中观察到柱状碳酸钙的形成。作者认为,胶质芽孢杆菌通过其风化作用及较强的吸附功能,利用其分泌的碳酸酐酶(CA)和溶解CO2在分解磷灰石的过程中促进了碳酸钙的形成。In order to study whether Bacillus mucilaginosus can promote the formation of calcium carbonate or not under certain conditions, this research adopted two commonly used basic media of Bacillus mucilaginosus . The one is the nitrogen medium, and the other is the nitrogen-free medium. The apatite mineral was the sole source of calcium, and the B. mucilaginosus was grown in different media to synthesis calcium carbonate. Scanning electron microscopy （SEM）, energy dispersive spectrometer （EDS） quantitative analysis, and X-ray diffraction （XRD） analysis were used to observe and study the crystal structure and chemical composition of the formed calcium carbonate. The results showed that B. mucilaginosus can accelerate formation of calcium carbonate. The crystals, calcium carbonate particles formed in the nitrogen medium containing apatite powders were more than those in the nitrogen-free medium containing apatite powders. Meanwhile, the columnar calcium carbonate crystals were observed in the nitrogen medium containing apatite powders. The mechanism for B. mucilaginosus to promote formation of calcium carbonate might be the secretion of carbonic anhydrase （CA） and dissolved CO 2 in the process of apatite weathering and strong absorption features of bacteria.

关 键 词：胶质芽孢杆菌 碳酸钙 生物成矿 诱导成矿 

分 类 号：Q939.99[生物学—微生物学]