狄氏剂对不同分化状态人多巴胺能SH-SY5Y细胞毒性的比较  

Cytotoxicity of Dieldrin to Human Dopaminergic SH-SY5Y Cells at Different Stages of Differentiation

作　　者：李芳芳[1] 王取南[1] 柴小玉[1] 包训迪[1] 高晔[1] 

机构地区：[1]安徽医科大学卫生毒理学系,安徽合肥230032

出　　处：《环境与健康杂志》2011年第3期192-195,I0001,共5页Journal of Environment and Health

基　　金：国家自然科学基金(30571585);安徽省自然科学基金(050430802)

摘　　要：目的比较狄氏剂对不同发育阶段的人多巴胺能SH-SY5Y细胞的毒性。方法以人多巴胺能SH-SY5Y细胞为模型,用10μmol/L全反式视黄酸(all-trans-retinoic acid,RA)分别诱导分化0、4和7 d,以1、3、10、30、100μmol/L的狄氏剂对细胞染毒24 h,以MTT法测定细胞存活率;按5×103个/孔的密度将细胞传至96孔板,用10μmol/L RA分别诱导分化0、4和7 d,以10、30、100μmol/L的狄氏剂对细胞染毒24 h,细胞活性/细胞毒性(LIVE/DEAD)试剂盒检测细胞的存活率和绝对死亡率。结果 MTT试验结果显示,狄氏剂可引起SH-SY5Y细胞存活率呈剂量依赖性下降(均P<0.01),与未分化(0 d)和已分化(7 d)的细胞相比,分化中(4 d)的细胞存活率在较低剂量(3μmol/L)就有明显下降(P<0.05)。细胞活性/细胞毒性染色结果显示,10～100μmol/L的狄氏剂可产生明显细胞毒性,SH-SY5Y细胞的存活率呈剂量依赖性降低,绝对死亡率呈剂量依赖性增高(均P<0.05)。已分化的细胞较未分化细胞的绝对死亡率更高(P<0.05),而分化中的细胞又比已分化细胞的绝对死亡率更高(P<0.05)。结论狄氏剂对不同发育阶段的多巴胺能神经细胞均有毒性作用,且分化中的细胞对狄氏剂的毒性作用更敏感。Objective To compare the cytotoxieity of dieldrin on dopaminergic neurons at different developmental stages. Methods All-trans-retinoic acid （10 μmol/L） was used to induce differentiation of SH-SYSY cells. MTr and Live/Dead viability/ cytotoxicity kit were used to assay the cytotoxicity of dieldrin to the undifferentiated （0 d）,differentiating （4 d） or differentiated （7 d） SH-SY5Y cells. Results The results of MTT showed that cells viability was decreased by dieldrin in a dose depended manner, The differentiating SH-SYSY cells were most sensitive to dieldrin. The results of LIVE/DEAD also showed that cells viability was decreased by dieldrin in a dose-dependent manner, The cells exposed to dieldrin at concentrations of 10-100 μmol/L were induced dose-dependent cell death. The differentiating/differentiated SH-SY5Y cells were more sensitive to dieldrin than the undifferentiated cells, and the differentiating SH-SYSY cells were more sensitive than the differentiated cells. Conclusion The dieldrin can induce cytotoxicity to dopaminergie neurons of different developmental stages,and the differentiating cells are most sensitive.

关 键 词：有机氯类农药 人成神经瘤细胞系 细胞分化 

分 类 号：R994.6[医药卫生—毒理学]