接种丛枝菌根真菌和有机土栽培对甜椒根际微生物分子多态性的影响  

Effect of Arbuscular Mycorrhizal Fungi and Organic Soil Substrate on the Microbial Molecular Diversity in Sweet Pepper Rhizosphere of Protected Cultivation

作　　者：王林闯[1] 贺超兴[1] 张志斌[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《生物技术通报》2011年第5期114-120,共7页Biotechnology Bulletin

基　　金：农业部行业科技项目(nyhyzx07-007);农业部园艺作物遗传改良重点开放实验室资助;中央级公益性科研院所基本科研业务费专项(082060302-05)

摘　　要：通过采用变形梯度凝胶电泳(DGGE)技术对普通土、普通土+GM、有机土和有机土+GM 4个不同处理下甜椒根际土壤微生物的多样性进行研究。结果表明,接菌处理使细菌的种类在数量上有所增加,其中有新的细菌类型的出现,也有一些细菌类型的减少和消失,一些共有细菌种类的数量也得到了丰富;应用有机土栽培有利于一些菌群的生长繁殖,有助于促成优势菌群的建立;对电泳图谱的相似性系数分析表明,有机土对细菌菌群多样性的影响强于接种菌根真菌处理;普通土接种菌根真菌对土壤微生物种群的影响强于有机土;接种菌根真菌使土壤的细菌种群结构发生了变化,并增加其菌群种类的相似性。The DGGE technique were used to study the effect of different treatments（general soil,general soil＋GM,organic soil,organic soil＋GM）on the microbial molecular diversity in sweet pepper rhizosphere.The results showed that the number of bacteria species increased with the inoculation of GM,which including the appear and disappear of some kinds of bacteria.The organic soils are beneficial to the growth and propagation of some bacterial flora,and make some advantage flora built up.Through analyses the similarity of DGGE profile can get that the effect of organic substrate is stronger than the inoculation of GM on soil microbial flora,and the general soil＋GM is stronger than organic soil.The structure of bacteria species in both soil have a same change with the inoculation of GM,which increased the similarity of their bacteria species.

关 键 词：丛枝菌根真菌 有机土 DGGE 微生物分子多态性 甜椒 

分 类 号：S641.3[农业科学—蔬菜学]