产葡萄糖酸荧光假单胞菌的分离鉴定及解磷作用  

Isolation and Identification of Gluconic-acid Producing Pseudomonas fluorescens and Phosphate Dissolution

作　　者：彭帅[1] 韩晓日[1] 马晓颖[1] 韩梅[1] 

机构地区：[1]沈阳农业大学土地与环境学院,沈阳110161

出　　处：《生物技术通报》2011年第5期137-141,共5页Biotechnology Bulletin

基　　金：国家产业技术体系花生专项(nycytx-19);辽宁绿丰农业技术开发中心合作项目(4130206-1103-01064508-02)

摘　　要：从番茄根际土壤中分离到1株解磷荧光假单胞菌LAD6,并对其进行了形态特征、生理生化鉴定及16S rDNA同源性序列分析。高效毛细管电泳仪(HPCE)分析结果显示,LAD6能够产生葡萄糖酸。对LAD6的解磷能力的测定结果表明,培养液中的磷溶解量的变化与菌株LAD6的生长和生理代谢的变化相关,其最大磷溶解量达到了24.5μg/mL。Bacterial strain LAD6 was isolated from tomato rhizosphere soil and identified by the characteristics of morphology,physiology and biochemistry tests and the comparison of 16S rDNA sequence.LAD6 can excrete gluconic-acid analysis by HPCE.The P-solubilizing of LAD6 was studied.The result showed that the change of the P-solubilizing content was correlated with the growth and metabolism of LAD6,and its highest P-solubilizing content was 24.5 μg/mL.

关 键 词：荧光假单胞菌 鉴定 葡萄糖酸 解磷 

分 类 号：S154.3[农业科学—土壤学]