微波和γ射线复合照射致Raji细胞损伤的形态学及凋亡观察  

Observation of Injury Effects and Apoptosis induced by Microwave and Gamma Ray on Lymphocyte in Raji Cell

作　　者：夏红杰[1,2] 王德文[2] 左红艳[2] 徐新萍[2] 贾锴[2] 邱滨涛[2] 

机构地区：[1]武警总医院中西医结合康复理疗科,北京100039 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《辐射防护》2011年第3期158-161,共4页Radiation Protection

基　　金：国家自然科学基金资助项目(30230360)

摘　　要：为了观察微波和γ射线复合照射对Raji细胞的损伤效应及细胞凋亡情况,应用微波和γ射线复合照射Raji细胞,用倒置相差显微镜观察照射前后细胞的形态学改变;采用Annexin-V和PI双标记检测细胞凋亡和坏死率的改变。结果表明,微波和γ射线照射均可造成淋巴细胞的形态改变并引起凋亡和坏死增加,复合照射对Raji细胞的损伤效应较单纯照射加重,损伤以γ射线损伤效应的特点为主要表现;各照射组细胞凋亡和坏死率的变化趋势与照射后细胞形态学的观察结果具有一致性。To investigate the rule of apoptosis, necrosis and the effects of Raji cell induced by microwave and gamma ray, the Raji cell was exposed to microwave radiation and gamma radiation. Morphological changes were observed by inverted phase contrast microscope before and after radiation. Annexin-V and PI double labelling were used to detect changes of apoptosis and necrosis rate. The results show that the cell shape was changed and the rate of apoptosis and necrosis were increased after exposure to microwave and γ ray. The injury effect of γ＋S-HPM compound radiation was more serious than any single radiation on lymphocyte. The major characteristics of injury showed as gamma ray effect. The trends of apoptosis and necrosis keep consistency with the change of the cell morphology after radiation between each observation group.

关 键 词：微波 Γ射线 复合照射 RAJI细胞 凋亡 

分 类 号：R82[医药卫生—临床医学]