不同含水量紫花苜蓿种子卫星搭载后植株叶片保护酶活性的研究  

Study on protective enzyme activities of alfalfa leaves from satellite carrying seeds with different moisture contents

作　　者：马学敏[1,2] 张治安[1] 邓波[2] 张蕴薇[2] 高洪文[3] 庞莹莹[2] 杨世超[2] 刘蒙[2] 

机构地区：[1]吉林农业大学,吉林长春130118 [2]中国农业大学动物科学学院草地研究所,北京100193 [3]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《草业科学》2011年第5期783-787,共5页Pratacultural Science

基　　金：国家科技支撑项目(2008BAD95B12);国家牧草产业技术体系--农业部牧草种质资源保护

摘　　要：利用"实践八号"育种卫星搭载含水量分别为9%、11%、13%、15%、17%的紫花苜蓿(Medicago sativa)品种"中苜一号"种子,以SP1第3年龄的植株为材料,分析其保护酶(SOD、CAT和POD)活性及丙二醛(MDA)含量。结果表明,不同种子含水量的紫花苜蓿植株的保护酶活性和丙二醛含量存在差异,地面对照组种子含水量为13%和15%的植株叶片保护酶活性均显著低于种子含水量为9%的植株叶片保护酶活性(P<0.05);种子含水量为13%的植株叶片MDA的含量显著高于种子含水量9%的植株(P<0.05)。卫星搭载组,高水分含量可以提高空间诱变效率,卫星搭载种子含水量为13%和15%的植株叶片的保护酶活性显著高于相应含水量地面植株叶片的及含水量9%的地面植株叶片的酶活性(P<0.05);卫星搭载种子含水量13%的植株叶片MDA含量比相应含水量的及含水量9%的地面植株叶片的MDA含量显著降低(P<0.05)。结果说明,空间诱变有效增强了SOD、CAT和POD的活性,减少了MDA的累积,降低了膜脂的氧化程度,增强了植株的抗氧化能力。Seeds with different moisture contents （9%, 11%, 13%,15%, 17%） of Medicago sativa cv. Zhongmu No. 1 were boarded on the Shijian-8 satellite. Using the age 3 of plants in SP1 as study materials, activities of leaf protective enzymes （SOD, CAT, POD） and MDA content were analyzed statistically. The results of the study showed that plant protective enzymes activities and MDA contents of alfalfa leaves from seeds with different moisture contents were different. The leaf protective enzyme activities with 13% and 15% seed moisture contents in control group were significant lower than that with 9% seed moisture content （P〈0.05）; the leaf MDA content with 13% seed moisture content was significant higher than that with 9% seed moisture content （P〈0.05）. In satellite carrying group, mutagenic efficiency of the seed with high moisture content could be increased. The leaf protective enzyme （SOD, CAT and POD） activities of satellite carried seed with 13% and 15% moisture content were significant higher than those of the seeds with same level of moisture content and 9% moisture content in control group （P〈0.05）. The leaf MDA content of satellite carried seeds with 13% moisture content was dropped comparing with those of the seeds with same level of moisture content and 9% moisture content in control group significantly （P〈0.05）. The results showed that the space mutagenesis enhanced the activities of SOD, CAT and POD, and reduced the accumulation of MDA and level of lipid oxidation, but the antioxidant capacity 9f plants was enhanced.

关 键 词：卫星搭载 紫花苜蓿 种子含水量 保护酶 

分 类 号：S541.9[农业科学—作物学]