东亚飞蝗染色体C带和银染带型分析　　  

Analysis on C-banding and silver staining karyotypes of Locusta migratoria manilensis

作　　者：吴学芳[1] 何正波[2] 陶红梅[1] 蔡志华[1] 

机构地区：[1]重庆师范大学生命科学学院动物学重点实验室,重庆400047 [2]重庆师范大学资源昆虫和分子生物研究所,重庆400047

出　　处：《草业科学》2011年第5期831-835,共5页Pratacultural Science

基　　金：国家自然科学基金(No.30700435)

摘　　要：本研究采用改进后的悬浮滴片法制备东亚飞蝗(Locusta migratoria manilensis)雄性染色体标本,进行东亚飞蝗核型、C带和银染显带分析。东亚飞蝗雄性核型为2N=22+XO(♂),染色体组式为4L+4M+4S+XO。C带显示东亚飞蝗染色体全部为端着丝粒,第2对染色体有端带;东亚飞蝗的银染核仁组织区位于2号染色体近端部次缢痕处,仅有1条染色体深染。2号染色体带型丰富,可作为东亚飞蝗的遗传标记。The improved dropping-method was used to make chromosome slides of male Locusta migratoria rnanilensis for studying the karyotypes of C-banding and sliver staining of L. migratoria manilensis. The result of C-banding showed that all chromomsomes of L. migratoria rnanilensis were telocentric chromosomes. Telomeric band was detected on chromosme 2. Karyotype of male L. migratoria manilensis was 2N=22＋XO（♂）. The genome-style was 4L＋4M＋4S＋XO. Ag-NOR was only observed on one of chromsome 2 and located on the secondary constriction near the telomere. Chromsome 2 had abundant bands and could be used as the genetic marker of L. migratoria manilensis.

关 键 词：东亚飞蝗 C带 银染核仁组织区 

分 类 号：S433.2[农业科学—农业昆虫与害虫防治]