在线酸坝富集-毛细管区带电泳法测定槐角中的染料木素  

Determination of genistein in fructus sophorae by capillary zone electrophoresis based on online acid barrage stacking

作　　者：冯海燕[1] 李向军[2] 袁倬斌[2] 

机构地区：[1]石家庄学院化工学院,石家庄050035 [2]中国科学院研究生院化学与化学工程学院,北京100049

出　　处：《分析试验室》2011年第5期30-33,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(20775088);河北省科技支撑计划项目(10212144);石家庄市科学技术研究与发展指导计划项目(09120851;石家庄学院博士科研启动基金项目(2009)资助

摘　　要：建立了分离测定槐角提取液中染料木素的在线酸坝富集-毛细管区带电泳方法,考察了酸的种类、酸的及浓度、进样/进酸时间比例等因素对堆积效率的影响。实验以20 mmol/L硼砂(pH10.5)作为缓冲溶液,200 mmol/L柠檬酸溶液(pH1.7)作为酸坝,进样、进酸时间分别为180s和45s,在分离电压15kV,检测波长254 nm的条件下,染料木素5 min内出峰,检出限可达10 nmol/L,富集倍数为300多倍。A method was established for analysis based on online acid bahage stacking. The of genistein in Fructus Sophorae by capillary zone electrophoresis effect of several factors （such as acid type, acid pH, acid concentration and the ratio of injection time of sample and acid） on stacking efficiency was investigated. Genistein was detected within 5 nfin under the following conditions：20 mmol/L borax （pH 10.5） as running buffer, 200 mmol/L citric acid（pH 1.7） as acid barrage, injection time of 180s for sample, 45s for acid, separation voltage of 15 kV, detection wavelength 254 nm. In this method, the limit of detection for genistein was 10 nmol/L with more than 300 fold accumulation concentration.

关 键 词：毛细管电泳 染料木素 酸坝堆积 在线富集 槐角 

分 类 号：O657.8[理学—分析化学]