HPLC法测定人尿中尿酸含量  

HPLC method for the determination of uric acid in human urine

作　　者：胡坪[1,2] 王瑞娟[1,2] 王月荣[1,2] 张敏[3] 章弘扬[1,2] 

机构地区：[1]上海市功能材料化学重点实验室,华东理工大学,上海200237 [2]华东理工大学化学与分子工程学院,上海200237 [3]华东理工大学药学院,上海200237

出　　处：《分析试验室》2011年第5期50-53,共4页Chinese Journal of Analysis Laboratory

基　　金：上海市科委攻关计划“扶正化瘀复方药效物质基础和多元释药系统的研究”项目(No.08DZ1971100)资助

摘　　要：建立了一种人体尿液中尿酸含量的高效液相色谱测定方法。采用ACE5AQ亲水色谱柱，pH3．2的乙酸水溶液为流动相，检测波长280nm。尿酸含量在7．1～224．6μg∥mL范围内线性关系良好，平均加样回收率为99．7％～100．5％，RSD小于1．4％。将该法用于健康人和肝硬化病人尿液样本的测定，两类样本中尿酸含量无显著性差异。A HPLC method was developed for the determination of uric acid in human urine. Urine samples were successfully separated on an ACE 5 AQ hydrophilic column with mobile phase of acetic acid solution （pH3.2）.The detection wavelength was set at 280 nm. The linear curve of uric acid concentration was within the range of 7.1 -224.6 pμg/mL and the average spiked recoveries were 99.7% - 100.5% with the RSD below 1.4%. The analysis results of urine samples showed that there was no significant difference in urinary uric acid concentration levels between healthy persons and liver cirrhosis patients. The established HPLC method is simple, fast, accurate, and reliable, and thus can be applied to the determination of urle acid in ,clinical urine samples.

关 键 词：屎酸 高效液相色谱法 尿液 定量分析 肝硬化 

分 类 号：O657.7[理学—分析化学]