重组人结缔组织生长因子对人成骨细胞膜型基质金属蛋白酶1及基质金属蛋白酶2表达的影响  

Effect of recombinant human connective tissue growth factor on the expression of membrane type-1 matrix metalioproteinase and matrix metalloproteinase 2 in human osteoblasts

作　　者：隋国良[1] 彭依群[1] 何玉玲[1] 翟木绪[1] 廖二元[1] 

机构地区：[1]中南大学湘雅二医院代谢内分泌研究所,长沙410011

出　　处：《中华内分泌代谢杂志》2011年第3期246-249,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金项目（30572078,30600661）

摘　　要：使用重组人结缔组织生长因子（recombinant human connective tissue growth factor,rCTGF）干预体外培养的人成骨细胞,发现rCTGF可呈时间-剂量依赖性地促进人成骨细胞膜型基质金属蛋白酶1及基质金属蛋白酶2的表达,200 ng/ml CTGF作用24～48 h达最大效果.rCTGF可明显增强p38丝裂原活化的蛋白激酶（p38-MAPK）磷酸化,p38-MAPK阻断剂SB 23058可阻断rCTGF上调膜型基质金属蛋白酶1及基质金属蛋白酶2的效应.Human osteoblast was treated with recombinant human connective tissue growth factor （rCTGF）. This experiment showed that rCTGF increased membrane type-1 matrix metalloproteinase and matrix metalloproteinase-2 protein expression in a dose- and time-depentent manner in human osteoblasts. rCTGF induced activation of p38 MAPK in human osteoblasts. p38 MAPK inhibitor SB23058 abrogated the effect of rCTGF on the expressions of membrane type-1 matrix metalloproteinase and matrix metalloproteinase-2 in human osteoblasts.

关 键 词：重组人结缔组织生长因子 成骨细胞 膜型基质金属蛋白酶-1 基质金属蛋白酶-2 

分 类 号：R346[医药卫生—基础医学]