胰岛素抵抗大鼠肝脏组织p-IRE—lα的表达及意义  

The expression and significance of p-IRE-1 α in rat liver with insulin resistance

作　　者：罗红飞[1] 裴丽娜[1] 都健[1] 刘佳[1] 郭宁宁[1] 林志楠[1] 张克莹[1] 

机构地区：[1]中国医科大学附属一院内分泌科,沈阳110001

出　　处：《中华内分泌代谢杂志》2011年第3期250-252,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：辽宁省科学技术计划项目（2009225029）;辽宁省教育厅科学技术研究项目（L2010597）

摘　　要：采用高脂饲料喂养并经高胰岛素-正常血糖钳夹技术评估,建立胰岛素抵抗大鼠模型.与正常饮食组相比,高脂饮食组大鼠血清游离脂肪酸和基础胰岛素水平升高（P＜0.05）,平均葡萄糖输注率（GIR60-120）降低（P＜0.01）,肝脏组织p-IRE-1α蛋白表达升高（P＜0.05）.并且肝脏组织p-IRE-lα蛋白表达与血清游离脂肪酸水平呈正相关（r=0.627,P＜0.05）.提示高脂饲料喂养可能通过升高血清游离脂肪酸水平,引起肝脏内质网应激,并通过p-IRE-1 α参与胰岛素抵抗的发生.Fed with high-fat diet and assessed by hyperinsulinemia-euglycemia clamp technique, rat models with insulin resistance were successfully induced. Compared with normal chow group （ NC ）, serum concentrations of free-fatty acids（FFAs） and baseline insulin in high-fat diet group（HF） was higher（ P〈0.05 ）, the average glucose infusion rate from 60 to 120 min（ GIR60-120 ） was lower（ P〈0.01 ）, and the expression of p-IRE-lα in the liver was higher（ P〈0.05 ）. Furthermore, the expression of p-IRE-1α in the liver was positvely correlated with the serum concentration of FFAs. All these data indicate that high-fat diet may induce endoplasmic reticulum stress in the liver by elevating serum concentration of FFAs, and may participate in the genesis of insulin resistance via p-IRE-1α.

关 键 词：胰岛素抵抗 大鼠 肝脏组织 磷酸化肌醇需求激酶-1α 

分 类 号：R587[医药卫生—内分泌]