齐墩果酸抑制肝癌细胞QGY增殖和诱导凋亡的作用研究  

Inhibition Proliferation and Induction Apoptosis Research of Oleanolic Acid on Hepatoma Cell Line QGY

作　　者：黄开顺[1] 朱链链[2] 刘丹[2] 邹黎[2] 陈志琼[1] 

机构地区：[1]重庆医科大学生命科学研究院,重庆400016 [2]重庆医科大学药学院药物分析教研室,重庆400016

出　　处：《世界科技研究与发展》2011年第2期318-321,共4页World Sci-Tech R&D

摘　　要：目的研究齐墩果酸对人肝癌细胞QGY增殖的作用及与细胞内钙离子浓度([Ca^(2+)]i)关系。方法将浓度分别为40、80、100μg/ml齐墩果酸作用肝癌H细胞QGY24 h后,DAP1染色,以荧光显微镜观察细胞形态变化;以11组不同浓度齐墩果酸(5～400 μg/ml)作用QGY细胞24 h后,用四甲基偶氮唑蓝(MTT)法检测QGY增殖情况;分别以不同浓度齐墩果酸(80、100、120 μg/ml)作用QGY细胞24 h后,流式细胞仪检测细胞周期改变、细胞凋亡率和[Ca^(2+)]i。结果细胞增殖被抑制并发生凋亡;不同浓度齐墩果酸能够抑制QGY细胞株增殖,且在5～120μg/mL范围内呈剂量依赖性,药物作用细胞24 h、48的IC_(50)分别为76.27μg/mL和66.56μg/mL;处理组细胞周期在S期产生阻滞、细胞内[Ca^(2+)]i较对照组显著增加,细胞凋亡率和[Ca^(2+)]i与药物浓度存依赖关系。结论齐墩果酸能够抑制肝癌细胞QGY增殖和诱导其凋亡;诱导凋亡可能与细胞内[Ca^(2+)]i增加有关。Objective To investigate the role of oleanolic acid on the proliferation of human hepatoma Hep3B cell and study the relationship with intracellular Ca2 ＋ concentration （ [ Ca2＋ ] i）. Methods After QGY cells were treated with oleanolic acid of 40,80 and 100μg/ml respectively for 24 h and stained with DAPI, morphology changes of QJY cell were observed by fluorescmme microscopy. After QGY cells were treated with 11 group oleanolic acid of different concentrations ranged from 5 to 400μg/ml for 24 h, growth inhibition rates were measured by the MTr method. After QGY cells were treated with oleanolic acid of 80,100 and 120μg/ml respectively for 24 h, cell cycle, apoposis percentage and [ Ca2＋ ] i were checked by tlow eytometry. Results Ceil proliferation was inhibited. The apoptosis occurred. The proliferation of QJY cells could be inhibited by oleanolic acid of different concentrations in a dose dependent range from 5 to 120 μg/mL. After QJY cells were treated with OA for 24 h and 48 h,ICs0 was 76. 27 and 66.56μg/mL,respectively, Cell cycle was terminated at S phase by oleanolic acid. Intracellular Ca2＋ concentration was significantly increased. At the meantime, apoptosis ratios and increases of intracellular [Ca2＋] i depended on the concentration of oleanolic acid. Conclusion Oleanolic acid may inhibit the growth of QJY cells and induce/be apoptosis by up-regulating intracellular calcium ion concentration.

关 键 词：齐墩果酸 肝癌细胞QGY 细胞周期 细胞凋亡 细胞内钙离子浓度 

分 类 号：R285[医药卫生—中药学]