DNA作为一种特异性刺激剂刺激淋巴细胞增殖的研究  

Plasmid DNA as a special stimular to stimulate lymphocyte proliferation

作　　者：吴琼[1] 孙英军[1] 张艳[1] 郑海学[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室,兰州730046

出　　处：《免疫学杂志》2011年第4期316-318,共3页Immunological Journal

基　　金：国家重点基础研究发展计划(2005CB23201);国家科技支撑计划(2006BAD06A03)

摘　　要：目的为了探讨质粒DNA体外刺激淋巴细胞的增殖状况,建立了一种方便可靠的评价豚鼠细胞免疫水平的试验方法。方法用羧基荧光素乙酰乙酸琥珀酰亚胺酯(CFSE)染色豚鼠全血,经植物血凝素(PHA)和质粒DNA刺激培养,利用流式细胞术分析细胞的增殖状况。结果豚鼠全血经PHA和DNA刺激,淋巴细胞增殖能力不同;未免疫组经DNA和PHA刺激后增殖的差异显著,免疫组差异不显著。DNA质粒在体内外均可作为刺激源刺激淋巴细胞增殖。结论建立了一种基于活细胞染料CFSE染色的豚鼠全血淋巴细胞增殖试验方法,可方便、快速、有效地评价细胞免疫水平。To investigate the level of lymphocyte proliferation stimulated by plasmid DNA in vitro,and to establish a convenient and reliable method to assess the level of cellular immunity in guinea pig,the whole blood of guinea pig was stained by Carboxyfluorescein diacetate succinimidyl ester（CFSE）,then stimulated by phytohemagglutinin（PHA） and plasmid DNA,and cultivated for 3 days.The cell proliferation was detected by flow cytometry.We observed that PHA and DNA stimulation could promote lymphocyte proliferation： the proliferation in non-immune group was significantly enhanced,while the inactivated vaccine immune group did not significantly changed,which indicate that plasmid DNA can be used as animmunogen to stimulate lymphocyte proliferation.Through this study,a live cell-based dye CFSE staining of guinea pig whole blood lymphocyte proliferation test method was established to evaluate the cellular immunity conveniently and effectively.

关 键 词：豚鼠淋巴细胞 细胞免疫 CFSE染色 淋巴细胞增殖 

分 类 号：R392.1[医药卫生—免疫学]