过敏性哮喘患者外周血T-bet、GATA-3、RORγt和FoxP3 mRNA的表达变化  

Expressions of T-bet,GATA-3,RORγt and FoxP3 mRNA in peripheral blood of patients with allergic asthma

作　　者：施宇衡[1,2] 时国朝[1] 马佳韵[2] 艾香艳[1] 朱海星[1] 万欢英[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院呼吸科,上海200025 [2]上海交通大学医学院附属第三人民医院呼吸科,上海201900

出　　处：《内科理论与实践》2011年第2期113-116,共4页Journal of Internal Medicine Concepts & Practice

基　　金：上海市基础研究重点项目(项目编号:08JC1415200)

摘　　要：目的:探讨特异性转录因子T-bet、GATA-3、RORγt和FoxP3 mRNA在过敏性哮喘患者外周血中的表达变化。方法:选择30例慢性持续期的屋尘螨过敏性哮喘患者,其中轻度哮喘17例、中重度哮喘13例,14名正常人作为对照。通过荧光实时定量PCR方法检测3组患者外周血单个核细胞(PBMC)中T-bet、GATA-3、RORγt和FoxP3mRNA的表达。结果:轻度和中重度哮喘患者的GATA-3 mRNA表达均显著高于正常人(均P<0.01)。中重度哮喘患者的RORγt mRNA表达明显高于正常人和轻度哮喘患者(均P<0.01)。T-bet和FoxP3 mRNA表达在3组间差异无统计学意义(均P>0.05)。结论:过敏性哮喘中GATA-3表达增加,中重度哮喘中RORγt表达增加,GATA-3和RORγt在哮喘发病中起着重要的作用。Objective To investigate the expressions of T-bet,GATA-3,RORγt and FoxP3 mRNA in peripheral blood of patients with allergic asthma.Methods Seventeen patients with mild persistent and 13 patients with moderate-severe persistent house dust mite allergic asthma and 14 healthy controls were enrolled in this study.Expressions of T-bet,GATA-3,RORγt and FoxP3 mRNA in peripheral blood mononuclear cell（PBMC） were determined by quantitative fluorescence PCR.Results Expression of GATA-3 mRNA in mild and moderate-severe asthma patients was significantly higher than that in normal controls（P0.01）.Furthermore,expression of RORγt mRNA in patients with moderate-severe asthma was significantly higher than that in mild asthma patients and normal controls（P0.01）.However,there was no difference in expressions of T-bet and FoxP3 mRNA among these three groups（P0.05）.Conclusions GATA-3 expression was increased in allergic asthma,and RORγt expression was increased in moderate-severe allergic asthma.GATA-3 and RORγt may play important role in the pathogenesis of allergic asthma.

关 键 词：过敏性哮喘 T-BET GATA-3 RORΓT FOXP3 

分 类 号：R562.25[医药卫生—呼吸系统]