核酸恒温扩增技术研究进展  

Advances in the Research of Nucleic Acid Isothermal Amplification Technologies

作　　者：汪琳[1] 罗英[1] 周琦[1] 赖平安[1] 柏亚铎[1] 

机构地区：[1]北京出入境检验检疫局检验检疫技术中心,北京100026

出　　处：《生物技术通讯》2011年第2期296-302,共7页Letters in Biotechnology

基　　金：国家"十一五"转基因重大专项(2008ZX08012-005)

摘　　要：核酸恒温扩增技术在生命科学研究及相关诸多领域已经得到了广泛应用。我们对核酸恒温扩增技术的最新进展作一简要综述,包括环介导恒温扩增、链替代扩增、依赖核酸序列的扩增、滚环扩增、切口酶核酸恒温扩增、依赖解旋酶的恒温扩增、转录依赖的扩增、杂交捕获法、转录介导的扩增等的原理、优缺点及应用。Nucleic acid isothermal amplification technology has been widely applied in life science research and related areas.In the paper,we briefly summarized the progress of nucleic acid isothermal amplification technology,which includes the theories,advantages and disadvantages and application of loop-mediated isothermal amplification,strand displacement amplification,nucleic acid sequence-based amplification,rolling circle amplification,nicking enzyme mediated amplification,helicase-dependent isothermal DNA amplification,hybrid capture,et al.

关 键 词：核酸恒温扩增 环介导恒温扩增 链替代扩增 依赖核酸序列的扩增 滚环扩增 

分 类 号：Q78[生物学—分子生物学]