纳米孔硅灰石载药抗菌止血材料的研究  

Hemostatic Agent of Nano Prorous Wollastonite Loaded Drug with Antibacterial Properties

作　　者：陈于[1] 徐灿[1] 苏佳灿[1] 李文锐[1] 

机构地区：[1]上海市第二军医大学附属长海医院,上海200433

出　　处：《无机化学学报》2011年第4期625-630,共6页Chinese Journal of Inorganic Chemistry

基　　金：上海市科委纳米专项(No.0852nm04300);上海市人事局人才发展基金(No.2009003);中国博士后科学基金面上项目(No.20100470756)资助

摘　　要：采用溶胶-凝胶法,以聚氧乙烯-聚氧丙烯-聚氧乙烯三嵌段共聚物(P123)为模板剂,合成了纳米孔硅灰石(np-WT),用np-WT载盐酸万古霉素研制了一种新型的载药抗菌止血材料,并对其止血性能进行了研究。结果表明:np-WT具有排列有序的纳米孔道结构,其孔径在2 nm左右,高比表面积的np-WT能够明显地缩短体外部分凝血活酶时间(APTT)和凝血酶原时间(PT)。载药np-WT能够在磷酸缓冲溶液(PBS)中缓慢地释放药物,载药np-WT对其凝血性能没有明显的影响。载药np-WT对大肠杆菌有很好的抗菌作用,细胞毒性实验表明:载药np-WT无细胞毒性。载药np-WT具有很好的止血性能,能够阻止兔背部伤口的流血和缩短其流血时间。A nanoprorous wollastonite（np-WT） was fabricated by using amphiphilic pluronic polymer surfactant（P123,EO20PO70EO20） as templates and sol-gel process,and a hemostatic agent of np-WT loaded drug（Vancomycin Hydrochloride） with antibacterial properties was investigated.The results showed that np-WT with large surface area and well ordered pores of around 2 nm could significantly shorten prothrombin time（PT） and the activated partial thromboplastin time（APTT）.The np-WT loaded drug had no obvious effects on its hemostatic properties.The np-WT could slowly release drug into phosphate buffered saline（PBS） for 7 d.The np-WT loaded drug without cytotoxicity had good antibacterial properties.The np-WT loaded drug could shorten the bleeding time of rabbit back wound and control hemorrhage,showing good hemostatic properties.

关 键 词：纳米孔硅灰石 止血 药物缓释 抗菌 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]