肝素杂化材料的制备及抗凝血性质的初步研究  

Preparation and Preliminary Study of Anticoagulant Properties of Heparin Hybrid Materials

作　　者：宋玉民[1] 缐萍[1] 李清萍[1] 张玉梅[1] 马新贤[1] 张强[2] 王怡云[2] 

机构地区：[1]西北师范大学化学化工学院,兰州730070 [2]甘肃省肿瘤医院,兰州730050

出　　处：《无机化学学报》2011年第4期631-636,共6页Chinese Journal of Inorganic Chemistry

基　　金：甘肃省自然科学基金(No.0710JRZA108);甘肃省高分子材料重点实验室资助项目

摘　　要：以甲苯二异氰酸酯(TDI)中的-NCO基与纳米金属氧化物表面的羟基发生反应,得到改性纳米金属氧化物,并使其与肝素钠(Heparin)进行接枝反应生成肝素杂化材料,结合红外、热重、扫描电镜(SEM)等表征方法,确定纳米金属氧化物确实接枝到了肝素钠的表面。通过对体外凝血时间和复钙时间的测定,来初步研究肝素杂化材料的抗凝血性质。结果表明:肝素杂化材料的抗凝血时间和复钙时间均比肝素钠的要短,表明它的抗凝血性比肝素钠的抗凝血性要弱一些;但比纳米金属氧化物和空白组的抗凝血时间和复钙时间要长,说明肝素杂化材料的抗凝血性与其相比则有明显的提高。Heparin hybrid materials were obtained by the grafting reaction of heparin and modified metal oxide nanoparticles,which were synthsized by the reaction between the TDIs NCO group and the hydroxyl group on the surface of nano metal oxides,and characterized by infrared spectra,thermogravimetry,scanning electron microscopy（SEM）.The anticoagulant effect of hybrid materials were studied by the measurements of coagulation time（CT） and recalcification time（RT）.The results showed that nano metal oxides were indeed grafted on the heparin.Compared to heparin,the coagulation time and recalcification time of heparin hybrid materials is shorter,but longer than the corresponding nano metal oxides and control group.In conclusion,the heparin hybrid materials were successfully prepared,and can shorten anti-coagulation time of heparin,prolong anti-coagulation time of nano metal oxides.

关 键 词：TDI 纳米金属氧化物 肝素 复钙时间 抗凝血性 

分 类 号：O614.121[理学—无机化学] O614.81[理学—化学]