nesfatin-1／NUCB2在人及鼠类动物消化系统的表达  

Distribution of nesfatin-1/NUCB2 in the digestive system of humans and rodents

作　　者：张爱青[1] 李学良[1] 蒋春樱[1] 林琳[1] 施瑞华[1] 

机构地区：[1]南京医科大学第一附属医院消化科,210029

出　　处：《中华消化杂志》2011年第2期112-116,共5页Chinese Journal of Digestion

基　　金：基金项目：国家自然科学基金（No.81070308/H0308）及中国杨森研究基金（2008）资助项目

摘　　要：目的 探讨一种新的摄食调节肽nesfatin-1/NUCB2在人、SD大鼠及ICR小鼠消化系统的分布,为进一步研究其在消化系统的功能奠定形态学基础.方法 取20例南京医科大学附属第一医院因消化系统疾病而行手术者的手术切除标本共27个,SD大鼠及ICR小鼠的胰腺、胃、十二指肠、食管、肝、小肠、结肠组织.其中恶性肿瘤患者标本为距肿瘤5 cm以上的癌旁组织,良性肿瘤患者的标本为病变边缘组织.免疫组化检测nesfatin-1/NUCB2的分布状况,Western印迹检测NUCB2蛋白的含量.结果 免疫组化显示nesfatin-1/NUCB2免疫阳性细胞在人、SD大鼠和ICR小鼠消化系统中的表达部位大致相似,主要分布于胰腺胰岛的中央;胃黏膜腺下1/3至中1/2的内分泌细胞及十二指肠黏膜下层的布氏腺中.在人、SD大鼠和ICR小鼠组织中均检测到了NUCB2蛋白的表达,但胰腺（分别为0.84±0.03、0.84±0.05和0.84±0.04）、胃（分别为0.86±0.06、0.81±0.02和0.78±0.02）及十二指肠（分别为0.79±0.09、0.79±0.04和0.78±0.05）处的NUCB2蛋白含量显著高于食管（分别为0.43±0.04、0.44±0.02和0.47±0.06）、肝（分别为0.42±0.01、0.44±0.04和0.43±0.01）、小肠（分别为0.32±0.04、0.32±0.04和0.34±0.04）及结肠（分别为0.29±0.01、0.32±0.03和0.28±0.03）中的蛋白含量（P值均=0.000）.结论 nesfatin-1/NUCB2这种新的摄食调节肽广泛存在于人、SD大鼠及ICR小鼠的胰岛、胃黏膜腺内分泌细胞及十二指肠布氏腺中,提示其在外周摄食调节、碳水化合物代谢及胃肠功能的调节方面存在一定的功能.Objective To investigate the regional distribution and morphological features of nesfatin-1/NUCB2 in digestive system of the humans, Sprague-Dawley （SD） rats and the institute of cancer research （ICR） mice, so as to lay the foundation for further study of its functions in the digestive system. Methods The specimens were obtained from SD rats and ICR mice as well as 20 patients with digestive disease, who were admitted to the First hospital affiliated to Nanjing Medical University and receired surgercal treatment. The specimens from patients with malignant tumors were obtained 5 cm apart from cancerous tissues and from patients with benign tumors were obtained near the focus. The resected tissues included pancreas, stomach, duodenum, esophagus, liver, small intestine or colon. The distribution of nesfatin-1/NUCB2 was examined with immunohistochemical （IHC） staining and its protein level in each organ was measured using Western blotting. Results The immuinohistocemical study revealed the similar distribution pattern of nesfatin-1/NUCB2 in the digestive system of the patients, SD rats and ICR mice. Nesfatin-1/NUCB2 was found to localize in the center of the pancreatic islets, the lower 1/3 to 1/2 of the gastric mucosal glands, as well as the submucosa of the duodenum. Western blotting examination showed the expression of NUCB2 in all tissues from patients, SD rats and ICR mice, whereas the protein level of the nesfatin-1/NUCB2 was higher in pancreas （0.84±0.03, 0. 84±0.05 and 0. 84±0.04, respectively）, stomach （0.86±0.06,0.81±0.02 and 0. 78±0.02, respectively） or duodenum （0.79±0.09,0. 79±0.04 and 0.78±0.05）than that in esophagus （0.43±0.04,0.44 ± 0.02 and 0.47 ± 0.06, respectively）, liver （0.42±0.01,0.44±0.04 and 0.43 ± 0.01, objectively）, small intestine （0.32±0.04,0. 32 ± 0. 04 and 0.34 ±0.04, respectively） or colon （0. 29±0.01,0.32±0.03 and 0. 28±0.03, respectively）（all P values=0. 000）. Conclusion Nesfatin-1/NUCB2 is widely expressed in the pancre

关 键 词：Nesfatin-1蛋白 Nucleobindin2蛋白 胰腺 胃 十二指肠 BRUNNER腺 免疫组织化学 

分 类 号：R346[医药卫生—基础医学]