云南玉溪烟区轮作与连作土壤细菌群落多样性比较研究  

Comparison of Soil Bacterial Diversity in Rotation and Monocropping Soil in Tobacco Growing Area in Yuxi,Yunnan,China

作　　者：贾志红[1,2,3] 易建华 苏以荣[1] 曾军英[4] 

机构地区：[1]中国科学院亚热带农业生态研究所,长沙410125 [2]中国科学院研究生院,北京100049 [3]湖南中烟工业有限责任公司,长沙410007 [4]湖南怀化学院,怀化418000

出　　处：《应用与环境生物学报》2011年第2期162-168,共7页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院知识创新工程重要方向项目(No.KSCX2-YW-N-48-1);湖南中烟工业有限责任公司科研项目(No.2007-YC-001)资助~~

摘　　要：为评价云南玉溪烟区轮作和连作对土壤细菌群落多样性影响,从土壤中直接提取土壤细菌总DNA,用细菌16S rDNA特异性引物进行PCR扩增和变性梯度凝胶电泳(DGGE),每个处理样品3次重复,在DGGE图谱中相似性较高,基本聚在一起,从整体证明了试验操作方法较为精确.从泳道分析图可知,轮作土壤细菌群落各泳道条带数量13～29条不等,平均为21条.连作土壤细菌群落各泳道条带数量从4～20条不等,平均为12条.从每条泳道的条带数方面和光密度值分别进行细菌群落多样性各指标的比较,结果表明,轮作处理细菌群落丰富度指数均大于连作处理,轮作香农-威纳和辛普森指数(1/D)均大于连作,表明轮作微生物多样性较连作高,轮作方式可以提高植烟土壤细菌群落的多样性.To evaluate the responses of soil bacteria community to two different tobacco cropping systems（rotation and monocropping）,soil bacteria were examined by culture-independent means,namely,16S rDNA PCR-DGGE profi ling.Crude DNA was extracted from the rotation and monocropping soil,amplied with bacterial specific primers of 16S rDNA by PCR,and separated by denaturing gradient gel electrophoresis（DGGE）.Subsequently,bands on DGGE were used to infer the diversity of bacteria in the rotation and monocropping soil.Three duplications of each treatment in the DGGE profile had higher similarity,almost getting together,showing the experiment premise for operation method.From the DGGE profile,the bacterial community bands in the rotation soil were found about 13～29,with average of 21,but the bands in the monocropping soil about 4～20,with average of 12.The comparison of the electrophoretic bands and optical density value with each index of bacterial richness and diversity showed the richness of the bacterial community in the rotation soil was higher than that in the monocropping soil.The Shannon-wiener index and Simpson index（1/D） of the rotation soil were all higher than those of the monocropping soil,which indicates that the rotation farming system can improve the bacterial diversity of soil.

关 键 词：轮作 连作 PCR-DGGE 土壤细菌群落 多样性 烤烟 

分 类 号：S154.36[农业科学—土壤学]