一株染料脱色酵母菌的鉴定及脱色特性  

Identification of a New Isolated Yeast and Its Decolorization on Dyes

作　　者：陈哈斯额尔敦 余志晟[1] 林文芳[1] 白志辉[1,3] 张洪勋[1] 

机构地区：[1]中国科学院研究生院资源与环境学院,北京100049 [2]平谷区农村工作委员会,北京101200 [3]中国科学院生态环境研究中心,北京100085

出　　处：《应用与环境生物学报》2011年第2期247-250,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.20877098);中国科学院研究生院院长基金项目(No.M3018);北京市科学技术委员会科技新星项目(No.2006A78)资助~~

摘　　要：从土壤中分离到一株活性艳红K-2BP脱色酵母菌株Y-63,根据其生理生化特征和26S rRNA基因序列相似性分析,鉴定为Pseudozyma rugulosa.该菌在16 h内对100 mg/L的活性艳红K-2BP脱色率为94%,其机理属于降解脱色.该染料脱色的最佳接种量(φ)应不低于5%,最适pH在4～9之间,(NH4)2SO4浓度(w)不低于0.1%,葡萄糖浓度(w)不低于0.2%.此外,该菌株对其它9种50 mg/L的染料(活性艳蓝X-BR、媒介漂蓝B、活性翠蓝KN-G、酸性媒介黄GG、媒介红S-80、依加仑蓝FBL 200%、弱酸艳红B、活性黑KN-B和活性红M-3BE)的脱色率在10%～96%之间.该研究表明酵母Y-63在染料废水的处理上具有较好的应用潜能.A new isolated yeast Y-63,capable of degrading Reactive Brilliant Red K-2BP,was obtained from soil,and identifi ed belonging to Pseudozyma rugulosa by its physio-biochemical characteristics and similarity analysis of 26S rRNA gene sequence.This strain gave a maximal decolorization（94%） for Reactive Brilliant Red K-2BP（100 mg/L） after incubation for 16 h.To get this decolorizing effect,the optimal inoculation volume（φ） of Y-63 was set not less than 5%,optimal pH of culture medium ranging from 4 to 9,and concentrations of（NH4）2SO4 and glucose not lower than 0.1% and 0.2%,respectively.In addition,it could also decolorize 10%～96% of other 9 dyes（Reactive Brilliant Blue X-BR,Mordant Blue B,Reactive Turquoise Blue KN-G,Acid Mordant Yellow GG,Acid Mordant Red S-80,Acid Blue FBL 200%,Weak Acid Brilliant Red B,Reactive Black KN-B and Acid Red M-3BE） of 50 mg/L.These results indicated that yeast Y-63 would have potential application in dye wastewater treatment.

关 键 词：菌种鉴定 活性艳红K-2BP PSEUDOZYMA rugulosa Y-63 脱色 废水处理 

分 类 号：X703[环境科学与工程—环境工程]