Single-molecule fluorescence imaging of membrane-bound proteins for studies of cell signal transduction  

作　　者：YANG Yong, XIA Tie ZHANG Wei FANG XiaoHong 

机构地区：[1]Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry,Chinese Academy of Sciences, Beijing 100190, China

出　　处：《Chinese Science Bulletin》2011年第11期1063-1067,共5页

基　　金：supported by the National Basic Research Program of China(2007CB935601);the National Natural Science Foundation of China(90713024,20821003);the Chinese Academy of Sciences

摘　　要：Fluorescence imaging of single molecules is becoming a powerful tool to examine biological processes at the molecular level.Using total internal reflection fluorescence microscopy (TIRFM),it has been possible to study the dynamic behavior of single molecules on living cell membranes.Herein,we briefly review the application of TIRFM-based single-molecule imaging in studies of membrane receptors involved in signal transduction.Furthermore,we discuss several examples of our own research on growth factor receptors,including TGF-β receptors,HER2,and EGFR,and speculate possible applications of this technique to investigate other cellular events occurring on or near the plasma-membrane.Fluorescence imaging of single molecules is becoming a powerful tool to examine biological processes at the molecular level. Using total internal reflection fluorescence microscopy （TIRFM）, it has been possible to study the dynamic behavior of single molecules on living cell membranes. Herein, we briefly review the application of TIRFM-based single-molecule imaging in studies of membrane receptors involved in signal transduction. Furthermore, we discuss several examples of our own research on growth factor receptors, including TGF-13 receptors, HER2, and EGFR, and speculate possible applications of this technique to investigate other cellular events occurring on or near the plasma-membrane.

关 键 词：细胞信号转导 单分子膜 荧光成像 结合蛋白 生长因子受体 荧光显微镜 单分子成像 生物过程 

分 类 号：Q257[生物学—细胞生物学]