用分光光度法连续测定血清中微量铜、锌的研究  

Continuous Determination of Trace Element Copperand Zinc in Human Serum by Spectrophotometry

作　　者：高晓钦[1,2] 李德芬[1,2] 谢吉民[1,2] 仲仁山[1,2] 

机构地区：[1]镇江医学院无机化学教研室 [2]镇江医学院卫生检验教研室

出　　处：《广东微量元素科学》1999年第7期44-46,共3页Trace Elements Science

摘　　要：研究了在ＴｒｉｔｏｎＸ－１００存在下，在ｐＨ８０的乙醇介质中，用５－Ｂｒ－ＰＡＤＡＰ作为显色剂，应用分光光度法连续测定血清中的铜、锌。本法具有较高的灵敏度，Ｃｕ２＋－５－Ｂｒ－ＰＡＤＡＰ的ε为８５０×１０４Ｌ·ｍｏｌ－１·ｃｍ－１，Ｚｎ２＋－５－Ｂｒ－ＰＡＤＡＰ的ε为９６５×１０４Ｌ·ｍｏｌ－１·ｃｍ－１。铜线性范围为０～０４５ｍｇ·Ｌ１，锌线性范围为０～０４０ｍｇ·Ｌ１。A method for continuous determination of trace element copper and zinc in human serum by spectrophotometry with Cu2+-5-Br-PADAP in pH 80 ethanol medium and the presence of Triton X-100 has been established.The sensitivity was good. (Cu2+-5-Br-PADAP) was 850104 Lmol-1cm-1 and (Zn2+-5-Br-PADAP) was 965104 Lmol-1cm-1.The calibration curves were linear in a concentration range of 0045 mgL1 for Cu and 0040 mgL1 for Zn.

关 键 词：分光光度法 血清 铜 锌 测定 显色剂 

分 类 号：R446.112[医药卫生—诊断学] Q592.1[医药卫生—临床医学]