阔口尖毛虫纤毛器微管的激光共聚焦显微镜观察  

Observation of Cortical Ciliature Microtubules by Laser Scanning Confocal Microscopy in the Oxytricha platystoma

作　　者：郭键[1] 张小翠[1] 翟楠[1] 倪兵[1] 陈季武[1] 顾福康[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《动物学杂志》2011年第2期65-69,共5页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30770238;31071875)

摘　　要：应用激光扫描共聚焦显微术显示经荧光紫杉醇标记的阔口尖毛虫(Oxytricha platystoma)口围带、波动膜、额腹横棘毛、左右缘棘毛等纤毛器的微管类细胞骨架。其口围带基部含小膜托架、托架间连接微管和小膜基部微管束,波动膜基部含发达的微管骨架网,口围带和波动膜后端的汇合处含有口底托架及口后微管束,额腹横棘毛和左、右缘棘毛基部含前纵微管束、后纵微管束、横微管束或周围微管束。结果表明,阔口尖毛虫的纤毛器基部微管具有其种的特异性,尤其是波动膜在其特长的纤毛列基部形成发达的微管骨架网,这种巨大型波动膜及其基部微管骨架在尖毛科乃至其他腹毛类纤毛虫中未见报道;且本文所见的纤毛虫口底托架及口后微管束可能是腹毛类纤毛虫中普遍存在的口纤毛器基部微管结构。The microtubular cytoskeleton in the cortical ciliature of the Oxytricha platystoma was visualized by direct fluorescent labeling of FLUTAX and laser scanning confocal microscopy.The distribution pattern of base-associated microtubules was found to be in accordance with that of adoral zone membranelles（AZM）,dulating membranes（UM）,frontal-ventral-transverse cirri（FVTC）,and left-right marginal cirri（L-RMC）.The microtubular cytoskeleton of the AZM comprised of membranelle brackets and its connected microtubules as well as the base-associated microtubules.The base-associated microtubules of the UM contained well-developed microtubule cytoskeletal net.The confluence of AZM and UM contained the oral-end bracket and postoral microtubules.The base-associated microtubules of the FVTC,L-and RMC contained anterior longitudinal microtubules（ALM）,posterior longitudinal microtubules（PLM） and transverse microtubules（TM） or radiate microtubules（RM）.The results show that cortical ciliature base microtubules of O.platystoma have their species specificity,such as,UM of the O.platystoma form well-developed microtubule net at the base of the special long cilia series.The structure of such large AZM,UM and the base-associated microtubule skeleton have not been reported in ciliates.In addition,the oral-end bracket and postoral microtubules may be prevalent in hyportrichia ciliate base of adoral ciliature microtubules.

关 键 词：激光扫描共聚焦显微术 阔口尖毛虫 纤毛器微管 基部附属微管 

分 类 号：Q954.4[生物学—动物学]