食物供应量对转红色荧光蛋白基因唐鱼生存和生长的影响  

Effects of Food Supply Level on Survival and Growth of Transgenic Tanichthys albonubes Expressing the Red Fluorescent Protein

作　　者：樊佳佳[1] 白俊杰[1] 简清[1] 叶星[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室,广州510380

出　　处：《动物学杂志》2011年第2期108-113,共6页Chinese Journal of Zoology

基　　金：国家高技术研究发展计划项目(No.2009AA10Z105)

摘　　要：选取雄性杂合子转红色荧光蛋白基因唐鱼(Tanichthys albonubes)(简称:转基因唐鱼)与雌性非转基因唐鱼交配产卵,出膜7 d后,在水温(25.0±2.0)℃条件下,选择健康仔鱼进行饱食(食物供应量高)、半饥饿(食物供应量中)和饥饿(食物供应量低)3种处理,结果显示:(1)出膜后7～72 d的唐鱼饱食组与半饥饿组死亡率无显著差异(P>0.05),但饥饿组唐鱼死亡率均显著高于饱食组和半饥饿组(P<0.01),表明食物供应量影响唐鱼的生存能力;(2)红色荧光蛋白基因在唐鱼体内表达,肉眼观察,最早出现在出膜后第30天。在出膜后的72 d,对3个处理组中没有表达红色荧光蛋白的唐鱼进行PCR验证,均没检测到沉默表达个体,此时3个处理组转基因唐鱼与非转基因唐鱼数量比均接近1∶1,符合经典的孟德尔单显性基因遗传模式,说明食物供应量多寡对转基因唐鱼与非转基因唐鱼生存影响效应是一致的;(3)出膜后28、42、57和72 d,对3个不同处理组的唐鱼幼鱼体长进行比较,结果均是饱食组>半饥饿组>饥饿组,且组间差异显著或极显著(P<0.05或P<0.01)。而对72 d唐鱼幼鱼3个处理组内转基因唐鱼和非转基因唐鱼体长进行统计分析表明,3个处理组内转基因唐鱼和非转基因唐鱼体长差异均不显著(P>0.05),表明食物供应量多寡对转基因唐鱼与非转基因唐鱼生长影响效应也是一致的。综上所述,食物供应量对唐鱼生存和生长是有影响的,但对转基因唐鱼和非转基因唐鱼影响效应是一致的,可见转基因唐鱼在食物供应量缺乏条件下并没有生存竞争优势。We assessed fitness of transgenic Tanichthys albonubes to food supply level using 7-days healthy larvae from crossing heterozygous transgenic T.albonubes expressing the red fluorescent protein to non-transgenic T.albonubes.The larvae were randomly divided into three groups： satiation group（high food supply level）,semi-starvation group（medium food supply level） and starvation group（low food supply level） under（25.0 ± 2.0） ℃.The results showed that：（1） the mortality rate of 72-days of larvae in satiation group was not significantly different from those in semi-starvation group（P 0.05）,but larvae in starvation group was significant difference from both satiation group and semi-starvation group（P 0.01）.It indicates that food supply level affect the mortality rate of T.albonubes.（2） The red fluorescent protein was initially expression at 30-days larvae with visual inspection.The PCR method was used to make sure that there was no recessive expression in the three treatments at 72-days larval.At this point the number of transgenic and non-transgenic T.albonubes was close to the 1 ∶1 ratio,and consistent with classical Mendelian inheritance model,which indicates that transgenic and non-transgenic T.albonubes are equally effected by food supply level.（3） The body length at 28-,42-,57-and 72-days larvae was satiation group semi-starvation group starvation group at significant or very significant level（P 0.05 or P 0.01）.The larvae body length of 72-days larvae has no significantly between transgenic and non-transgenic ones within the same food supply level group.In summary,the food supply level had similar influence on survival and growth of T.albonubes for both transgenic and non-transgenic.We speculate that transgenic T.albonubes have no competition advantage in the low food availability.

关 键 词：转基因唐鱼 食物供应量 适合度 死亡率 

分 类 号：Q958[生物学—动物学]