青年人群A型行为与儿茶酚胺/核因子κB/促炎因子通路的关系  

A study of the association between type A behavior pattern and activity of catecholamine/NF-κB/pro-inflammatory cytokines in young persons

作　　者：宁淑娥[1,2] 曲鹏[1] 魏刚[1] 宗大飞[1] 张兴[1] 隋政 郑美丽[1] 

机构地区：[1]大连医科大学第二临床学院心管内科,116013 [2]海军大连舰艇学院心理咨询中心 [3]大连市甘井子区政府信访局

出　　处：《中华高血压杂志》2011年第3期251-256,共6页Chinese Journal of Hypertension

基　　金：国家自然科学基金资助项目(30670836)

摘　　要：目的探讨青年A型行为与儿茶酚胺/核因子κB(NF-κB)/促炎因子通路的关系,为阐明A型行为与心血管疾病关系提供依据。方法以明尼苏达多相人格调查表2(MMPI-2)A型行为量表评分将900名大一新生(男476人;女424人)分为A型(≥11分,159人)、中间型(6～10分,539人),B型(≤5分,202人)3组;采用酶联免疫吸附法检测血清肾上腺素、去甲肾上腺素、白细胞介素(IL)6、IL-18水平。随机在A型、B型、中间型行为者中各抽取8例(男女各4例),抽取各例外周血20mL,分成5组,分别在5组中加入多巴酚丁胺0、20、30、40和50μmol/L孵育18h后测定各组血清中IL-6和IL-18浓度,然后提取单核细胞用免疫组化法测定NF-κB蛋白表达变化。结果血清儿茶酚胺浓度与A型行为人格特征呈正相关(r=0.375～0.662,P<0.01);A型组血清肾上腺素、去甲肾上腺素高于中间型组和B型组;A型行为人格特征与血清IL-6和IL-18浓度呈正相关(r=0.294～0.778,P<0.05或P<0.01)。多巴酚丁胺呈剂量依赖性诱导人单核细胞NF-κB/促炎因子通路激活,以40μmol/L多巴酚丁胺为最适宜剂量;40μmol/L多巴酚丁胺诱导激动后,A和B型行为者的单核细胞NF-κBp65及血清IL-6和IL-18均明显上调(P<0.01或P<0.05);A型行为者的单核细胞NF-κBp65及血清IL-6和IL-18均高于B型行为(P<0.01)。结论 A型行为者非应激与应激时借助血清中较高浓度的儿茶酚胺诱导NF-κB/促炎因子通路持续处于激活状态,这可能是青年A型行为者的一个病理生理学特征。Objective Nuclear factor-κB（NF-κB）and pro-inflammatory cytokines play an important role in initiation and progression of atherosclerosis and coronary disease,type A behavior pattern（TABP）is lifestyle risk factor.The aim of the study was to investigate the association between TABP and activity of/NF-κB/pro-inflammatory cytokines signaling in young persons,and to explore the possible role of catecholamine in inducing activation of NF-κB/pro-inflammatory cytokines.Methods According to the MMPI-2 type A scale scores,900 college freshmen（male 476,female 424）were divided into type A（≥11,n=159）,type middle（6-10,n=539）,type B（≤5 scores,n=202）3 groups.The concentrations of serum norepinephrine（NE）,epinephrine（E）,pro-inflammatory cytokines [interleukin（IL）6,IL-18] in all subjects were measured with ELISA.8 subjects（male 4,female 4）were randomly selected from type A,type B and type middle group,respectively.20 mL peripheral blood sample collected from each subject was divided into 5 groups.The 5 groups were then incubated with various concentrations of Dobutamine（0,20,30,40,50 μmol/L）for 18 hours.The concentrations of serum IL-6 and IL-18 were measured by ELISA and NF-κB expression by immunohistochemistry.Results The concentration of serum E,NE and IL-6,IL-18 in type A group was significantly higher than that in group middle and in type B group（P0.01）.The significant association were found among E,NE and TABP total scores,TABP subscale scores and also IL-6,IL-18（r=0.294-0.778,P0.05 or P0.01）.After Dobutamine inducing,NF-κB expression as well as IL-6 and IL-18 concentrations were upregulated in a dose-dependent manner（P0.05 or P0.01）.Both NF-κB and IL-6,IL-18 in type A group were significantly higher than in type B group（P0.01）.Conclusion NF-κB/pro-inflammatory cytokines signaling is always activated during stress or not in young persons with TABP,catecholamine play a mediator role in inducing activation of the signaling.

关 键 词：A型行为 儿茶酚胺 核因子B 生活方式 危险因素 

分 类 号：R54[医药卫生—心血管疾病]