正弦波电磁场激活一氧化氮信号通路促进大鼠成骨细胞成熟与矿化  

Sinusoidal Electromagnetic Fields Enhance Rat Osteoblast Differentiation and Mineralization by Activating Nitric Oxide Signaling Pathway

作　　者：程国政[1] 葛宝丰[1] 陈克明[1] 李志锋[1] 周建[1] 韦哲[2] 

机构地区：[1]兰州军区兰州总医院骨科研究所,兰州730050 [2]兰州军区兰州总医院医学工程科,兰州730050

出　　处：《生物化学与生物物理进展》2011年第4期331-337,共7页Progress In Biochemistry and Biophysics

基　　金：甘肃省科技计划资助项目(092NKDA025)~~

摘　　要：研究正弦波电磁场(SEMF)促进成骨细胞成熟矿化是否与一氧化氮(NO)信号通路相关.首先检测成骨细胞经正弦电磁场作用0h、0.5h、1h、1.5h、2h、2.5h、3h、3.5h和4h后一氧化氮合酶(NOS)的活性,以探明电磁场是否影响NO的合成;其次,在细胞培养液中加入NOS的阻断剂L-NAME以阻断NO信号通路,观察电磁场促进骨形成作用是否受到影响.结果发现,经正弦波电磁场处理后,NOS活性升高,在0.5h达到峰值,与空白对照组差异极显著(P<0.01),Osterix基因的表达量、碱性磷酸酶活性和钙化结节数等均显著高于对照组.L-NAME组各项指标均低于空白对照组,SEMF+L-NAME组则略高于空白对照组而低于SEMF组.以上结果表明SEMF促进成骨细胞成熟矿化过程中NO信号通路被激活,如该通路被抑制,则SEMF的促成骨作用被抵消.It was investigated whether the effects of sinusoidal electromagnetic field（SEMF） stimulation on the differentiation and mineralization of osteoblast are mediated by the nitric oxide（NO） signal pathway.It was first investigated whether SEMF had an effect on nitric oxide synthase（NOS） activity measured on the 0 h,0.5 h,1 h,1.5 h,2 h,2.5 h,3 h,3.5 h,4 h after SEMF treatment.Secondly,the NOS inhibitor,L-nitro-arginine-methylester（L-NAME） was added to the culture medium to observe whether it inhibit the maturation and mineralization of osteoblast stimulated by SEMF,which evaluated by measuring alkaline phosphatase（ALP） activity,CFU-FALP,osterix gene expression and mineralized bone modulus.After treatment of SEMF,the NOS activity in SEMF group increased in comparison with the normal control group（P 〈 0.01）,reaching the highest level after 0.5 h.The gene expression of Osterix,ALPase activity and mineralized bone modulus in the SEMF group were also increased significantly.However,these effects were partially blocked in the L-NAME group.Surprisingly,all the osteogenic markers in the SEMF＋L-NAME group were slightly higher than those of the control group,but lower than those of the SEMF group.In conclusion,The NO signal pathway was activated by SEMF treatment.The stimulatory effects of SEMF on the differentiation and mineralization of osteoblast were attenuated when NO signal pathway was blocked.

关 键 词：正弦波电磁场 成骨细胞 分化 成熟 

分 类 号：Q64[生物学—生物物理学] R244[医药卫生—针灸推拿学]