Wnts及其相关分子在小鼠外胎盘锥中的表达  

Expressions of Wnts and Their Related Molecules in Mouse Ectoplacental Cone

作　　者：彭莎[1,2] 陈家欢[1,2] 曹选红[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100 [2]陕西省干细胞工程技术研究中心陕西省农业分子生物学重点实验室,杨凌712100

出　　处：《农业生物技术学报》2011年第2期314-322,共9页Journal of Agricultural Biotechnology

基　　金：西北农林科技大学08应届博士科研启动费(No.01140406)资助

摘　　要：本研究通过RT-PCR的方法检测了妊娠第8天小鼠(Mus musculus)外胎盘锥(ectoplacental cone,EPC)中Wnts及其相关分子mRNA的表达,并用免疫荧光化学的方法检测了经典Wnt通路关键分子β-catenin及此通路抑制因子Dkk1在体外培养的EPC中的蛋白表达,通过EPC体外扩展实验初步研究了经典Wnt信号通路在滋养层侵入中的作用。结果显示,Wnt家族中的11个成员、Frizzled受体家族的10个成员、Wnt家族的抑制剂sFRP家族的5个成员、Dkk基因家族和受体kremen的mRNA在小鼠EPC中都有不同程度的表达。β-catenin在从体外培养的EPC迁移出的滋养层细胞核中有强烈表达,而Dkk1不表达,用经典Wnt信号通路抑制因子Dkk1处理EPC后,滋养层细胞扩展直径增加,这表明经典的Wnt信号通路可能抑制滋养层侵入。本研究为揭示Wnt信号通路在胎盘建成的滋养层侵入过程的作用和机制提供理论基础。In this study,we detected gene expressions of Wnts and their related molecules in ectoplacental cone（EPC） from 8-day p.c.pregnant mouse（Mus musculus） uterus based on RT-PCR.The localizations of β-catenin（the key molecule of canonical Wnt pathway） and Dkk1（an inhibitor of Wnt pathway） were determined by immunofluorescence in EPC.We also primarily studied the function of canonical Wnt pathway during the trophoblast invasion by EPC outgrowth in vitro.Our results showed that 11 of 19 Wnt molecule members,10 Frizzled receptors,5 sFRP members（inhibitors of Wnt pathway）,Dkk and kremen1/2 were all expressed in mouse EPC.The β-catenin had strong expression in nucleus of trophoblast cells which were derived from the cultured EPC fraction.The outgrowth diameter of trophoblast increased after treatment of Dkk1,suggesting that the canonical Wnt pathway possibly inhibits the trophoblast invasion.Our study provided a theoretical basis for revealing the role of Wnt signaling pathway in the process of trophoblast invasion during murine placentation.

关 键 词：WNT通路 外胎盘锥(EPC) 滋养层侵入 

分 类 号：Q2[生物学—细胞生物学]