新疆多浪羊Fec^B突变检测及与产羔数的关系  

Detection of Fec^B Mutation and Its Relationship with Litter Size in Xinjiang Duolang Sheep(Ovis aries)

作　　者：史洪才[1,2] 高志英[3] 牛志刚[1] 白杰[1] 冯利君[2] 李红波[3] 贾斌[2] 张扬[3] 

机构地区：[1]新疆畜牧科学院农业部家畜繁育生物技术重点开放实验室,乌鲁木齐830000 [2]新疆石河子大学动物科技学院,石河子832003 [3]新疆畜牧科学院畜牧科学研究所,乌鲁木齐830000

出　　处：《农业生物技术学报》2011年第2期330-334,共5页Journal of Agricultural Biotechnology

基　　金：新疆维吾尔自治区“十一五”科技重大专项(No200731135);国家自然科学基金(No30760161);新疆维吾尔自治区科技支疆项目(No200891102)共同资助

摘　　要：骨形态发生蛋白受体IB基因(BMPR-IB)FecB突变是影响布鲁拉美利奴和小尾寒羊高繁殖力的主效基因。本研究采集产双羔及以上的多浪羊(Ovisaries)母羊135只及后代羔羊血样共353份,公羊血样211份,利用PCR-RFLP技术检测FecB突变,并分析不同基因型个体母羊的产羔数。结果表明:在多浪羊群体中检测到BB、B+和++基因型,基因型频率分别为0.005,0.146和0.849。B+型多浪羊产羔数均值比++型多0.51只(P<0.01),BB型多浪羊产羔数分别与B+和++型差异不显著。多浪羊群体在FecB位点上处于Hard-Weinberg平衡状态,表明在该位点上没有受到选择的影响。本研究首次在新疆多浪羊群体中发现了FecB突变BB基因型公、母羊个体(1只公羊和2只母羊),揭示多浪羊多羔产生的机理可能与布鲁拉美利奴和小尾寒羊相同,在生产过程中可以根据携带FecB突变的情况开展选种选配,组建多浪羊肉用高繁品系。FecB mutation of bone morphogenetic protein receptor IB gene（BMPR-IB） is the major gene responsible for prolificacy in Booroola Merino and Small Tail Han sheep.In our research,135 ewes with their two or more litters（totally 353） and 211 sires were selected.FecB mutation was detected by PCR-RFLP and the litter size of ewes with different genotypes were analyzed.The result showed that three genotypes（BB,B＋ and ＋＋） were detected in Duolang sheep（Ovis aries）.Genotype frequency of BB,B＋ and ＋＋ was 0.005,0.146 and 0.849,respectively.The Duolang ewes with genotype B＋ had 0.51（P0.01） lambs more than those with genotype ＋＋.No significant difference in ewes with genotype BB and B＋ or BB and ＋＋.Chi-square test showed that the Duolang sheep population was in Hardy-Weinberg equilibrium in FecB locus,which indicated that the population could not be affected by selection.It is the first time that BB genotype（one sire and two ewes） in FecB mutation was detected in Duolang sheep which reveals that the mechanism of high prolificacy in Duolang sheep can be the same as Booroola Merino and Small Tail Han sheep.In Duolang sheep industry,FecB mutation detection can be used to breed new strain of mutton and prolificacy.

关 键 词：多浪羊 多羔 FecB突变 

分 类 号：S826.92[农业科学—畜牧学]