两种检测方法分析慢性乙型肝炎患者血清白细胞介素-21水平的差异  

Serum concentration of IL-21 analyzed with two different immunological assays in chronic hepatitis B patients

作　　者：黄璇[1] 李咏茵[1] 胡小雄[1] 张明霞[1] 唐利波[1] 马世武[1,2] 侯金林[1] 

机构地区：[1]南方医科大学南方医院感染内科,广州510515 [2]成都军区昆明总医院感染科

出　　处：《中国病毒病杂志》2011年第1期41-44,共4页Chinese Journal of Viral Diseases

基　　金：国家自然科学基金资助项目(30730082;30901271);南方医院院长基金(2009C009)

摘　　要：目的比较两种常规免疫学检测方法检测慢性乙型病毒性肝炎(CHB)患者血清白细胞介素-21(IL-21)表达水平的差异。方法选择24例CHB患者(HBV DNA≥5 lg拷贝/ml、ALT≥40 U/L)以及12例健康献血者(HBsAg阴性、ALT<40 U/L),应用酶联免疫吸附试验(ELISA)和细胞计数微球芯片技术(CBA)检测血清IL-21的水平,其中20份血清同时进行了两种方法检测。结果 ELISA法检测发现,CHB患者血清IL-21平均浓度为(96.4±9.2)ng/L,12例健康对照者为(27.9±7.5)ng/L,两组间差异有统计学意义(P=0.000 5),HBeAg阳性[(88.9±9.3)ng/L,n=12]和HBeAg阴性[(103.9±16.0)ng/L,n=12]CHB患者血清IL-21浓度差异无统计学意义(P=0.665 0),但均显著高于健康人群[(27.9±7.5)ng/L,n=12](P=0.005 1,P=0.001 7)。ELISA法检测结果显著高于CBA法[(96.0±10.2)ng/Lvs(68.2±6.1)ng/L,P=0.013 0]。结论选择不同的方法检测血清IL-21浓度存在一定的差异;CHB患者血清高水平表达IL-21可能与慢性乙型肝炎的发病机制有关。Objective To measure the concentration of serum interleukin-21（IL-21）in chronic hepatitis B（CHB） patients with two different immunological assays.Methods Twenty-four CHB patients（HBV DNA≥105 copies/ml,ALT≥40 U/L） and 12 healthy volunteers participated the study.The serum concentration of IL-21 was analyzed by an enzyme linked immunosorbent assay（ELISA） and/or a cytometry beads array（CBA） test.Results There were 12 CHB patients with positive HBeAg testing result.The serum concentration of IL-21 was significantly higher in CHB patients [（96.4±9.2）ng/L,ELISA] than that in healthy volunteers [（27.9±7.5）ng/L,ELISA]（P0.05）.There was no difference in the serum concentrations of IL-21 between HBeAg-positive[（88.9±9.3） ng/L,n=12]and negative[（103.9±16.0）ng/L,n=12]CHB patients.The value of IL-21 was significantly higher by the method of ELISA assay[（96.0±10.2）ng/L]than that by CBA test[（68.2±6.1）ng/L]（P=0.013 0）.Conclusions CHB patients had a higher level of IL-21 expression than healthy volunteers.The difference might be associated with the pathogenesis of chronic hepatitis B.The value of serum IL-21 concentration was significant higher by the assay of ELISA than that by the method of CBA.

关 键 词：肝炎病毒 慢性 乙型 T淋巴细胞 白细胞介素-21 

分 类 号：R512.62[医药卫生—内科学]