非标记液晶型免疫传感器检测赭曲霉素A  

Detection of Ochratoxin A by Label-Free Liquid Crystal Immunosensor

作　　者：杨胜园[1,2] 谭慧[1] 刘艳梅[1] 吴朝阳[1] 沈国励[1] 俞汝勤[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室,长沙410082 [2]南华大学公共卫生学院卫化检测教研室,衡阳421001

出　　处：《高等学校化学学报》2011年第5期1065-1069,共5页Chemical Journal of Chinese Universities

基　　金：国家"九七三"计划项目(批准号:2007CB310500);国家"八六三"计划项目(批准号:2009AA063004)资助

摘　　要：研制了一种基于液晶取向改变的非标记液晶型免疫传感器,并用于检测赭曲霉素A(OTA).采用戊二醛交联法将OTA固定在由自组装膜修饰的玻璃基底表面.自组装膜能诱导液晶分子垂直排列,而连接OTA抗体后则扰乱了液晶分子取向的有序排列,导致液晶分子在化学敏感膜表面的取向发生变化,使光学信号的亮度及色彩发生变化,以此实现对OTA的检测.当OTA含量超过0.01 ng/mL时,光学信号发生明显变化.本方法具有灵敏度高、特异性好、非标记和操作简单等特点.A novel liquid crystal immunosensor based on the indirect competitive assay format was developed for the detection of ochratoxin A（OTA）.OTA was tethered onto the self-assembled film surface on a glass slide via a cross-linker glutaradehyde.The specific binding of anti-OTA antibody and ochratoxin A induced the homeotropic-to-tilted transition of LCs,resulting in the corresponding changes of opitical images under the crossed polarized light.The LC-based imaging method had a good signal-to-noise ratio and a clear distinction between positive and negative results.When the concentration of analyte OTA exceeded a critical value（0.01 ng/mL）,the opitical images showed a very strong response.The proposed sensor exhibited high sensitivity and desirable selectivity,and was label free and easy in operation.

关 键 词：液晶 自组装膜 免疫传感器 赭曲霉素A 

分 类 号：O657.39[理学—分析化学]