电活性可生物降解纳米复合材料PAP/op-HA/PLGA的制备及成骨活性  

Preparation and Osteogenesis Activities of Electroactive Biodegradable Nanocomposites PAP/op-HA/PLGA

作　　者：邬海涛[1,2] 于婷[3] 朱庆三[2] 焦自学[1] 危岩[4] 章培标[1] 陈学思[1] 

机构地区：[1]中国科学院长春应用化学研究所,高分子物理与化学国家重点实验室,长春130022 [2]吉林大学第一医院,长春130021 [3]吉林大学第二医院,长春130041 [4]清华大学化学系,北京100084

出　　处：《高等学校化学学报》2011年第5期1181-1187,共7页Chemical Journal of Chinese Universities

基　　金：国家“八六三”计划项目(批准号:2007AA03Z320);国家自然科学基金面上项目(批准号:50673090)资助

摘　　要：以苯胺五聚体(AP)与聚乳酸(PLA)的三嵌段共聚物(PAP)与表面接枝低聚乳酸的纳米羟基磷灰石(op-HA)和聚丙交酯-乙交酯(PLGA)的复合物共混,制备了电活性可降解纳米复合材料PAP/op-HA/PLGA,采用紫外-可见光谱、循环伏安扫描和标准四探针法分析其电化学特性及电导率.采用ESEM观察其膜表面形貌,用接触角评价其亲水性.通过在材料膜表面接种兔成骨细胞进行体外培养,采用荧光染色、NIH ImageJ图像分析和Real-time PCR综合评价细胞在材料表面的黏附、扩展(细胞面积比)和成骨相关基因的表达水平,以此评价新型电活性纳米复合骨修复材料PAP/op-HA/PLGA的表面性质和生物活性.结果表明,PAP/op-HA/PLGA的电导率较低(5×10-6 S/cm),但具有良好的电化学氧化还原性能,PAP质量分数为0.1%时,材料的亲水性明显改善,成骨细胞的黏附和扩展明显增强.培养7 d时骨形态蛋白-2(BMP-2)和骨连接蛋白(Osteonectin)的基因表达水平明显提高,而对Ⅰ型胶原蛋白的基因表达无明显影响.表明PAP/op-HA/PLGA具有良好的细胞相容性和成骨活性.The electroactive biodegradable nanocomposites PAP/op-HA/PLGA were prepared by blending a triblock copolymer PLA-b-AP-b-PLA（PAP） of polylactide（PLA） and aniline pentamer（AP） with oligomeric lactic acid-surface grafted hydroxyapatite（op-HA）/poly（lactide-co-glycolide）（PLGA）.The electrochemical properties and conductivity were characterized using UV-Vis spectroscopy,cyclic voltammetry and standard four-probe method.The film＇s surface morphology was displayed by ESEM and the contact angle was mea-sured to evaluate the film＇s hydrophilicity.Rabbit＇s osteoblasts were cultured in vitro on the film surface.For comprehensively evaluating surface properties and biological activity of the new electroactive nanocomposites PAP/op-HA/PLGA for bone repair,cell adhesion and cell spreading（cell area fraction） were determined using fluorescein isothiocyanate dyeing and NIH Image J software analysis,and gene expression levels were detected using real-time PCR.The results show that PAP/op-HA/PLGA composites have good electrochemical redox property and low conductivity（～5×10-6 S/cm）.With the mass fraction of 0.1% PAP,the hydrophilicity of composites was improved,the adhesion and expansion of osteoblasts were enhanced,and bone morphogenetic protein-2（BMP-2） and bone junction protein（osteonectin） gene expression levels were increased,significantly.In conclusion,PAP/op-HA/PLGA composites have good biocompatibility and osteogenic activity.

关 键 词：苯胺五聚体 嵌段共聚物 纳米羟基磷灰石 聚丙交酯-乙交酯 导电高分子 成骨活性 

分 类 号：O631[理学—高分子化学]