鲍氏不动杆菌耐药性及相关耐药基因检测分析  

Detection of resistance related genes of acinetobacter baumannii and antibiotics resistance

作　　者：张晓兵[1] 府伟灵[1] 

机构地区：[1]第三军医大学第一附属医院检验科,重庆400038

出　　处：《中华医院感染学杂志》2011年第10期1945-1947,共3页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金(30927002);全军医药卫生科研基金(06G073;08G089);国家计生委课题(2009-GJKJS-017);国家传染病重大专项分题(2008ZX10003-02;2008ZX10003-012);第三军医大学第一附属医院创新基金(SWH2007017)

摘　　要：目的检测分析鲍氏不动杆菌耐药性及相关耐药基因。方法采用K-B法(CLSI 2008年标准)测定鲍氏不动杆菌临床分离株耐药性;PCR方法检测TEM、SHV、CTX-M、I MP、VI M、OXA-23、OXA-24耐药基因以及Ⅰ、Ⅱ类整合酶。结果 130株鲍氏不动杆菌,除阿米卡星和碳青霉烯类外,对其他抗菌药物耐药率非常高,TEM、SHV、CTX-M、Int1、Int2基因阳性率分别为68.5%、10.8%、17.7%、75.4%、0;51株耐碳青霉烯类鲍氏不动杆菌中I MP、VI M、OXA-23、OXA-24基因阳性率分别为25.5%、19.6%、72.5%、0。结论鲍氏不动杆菌耐药性较高,携带TEM型β-内酰胺酶、OXA-23、I MP和VI M型碳青霉烯酶基因和Ⅰ类整合酶;产生OXA-23型碳青霉烯酶和金属酶是医院鲍氏不动杆菌对碳青霉烯类耐药的主要机制。OBJECTIVE To detect and analyze the antibiotic resistance and resistance related genes from Acinetobacter baumannii.METHODS Antimicrobial susceptibility test was done on A.baumannii clinical isolated by Kirby-Bauer method.Results were assessed according to the standards recommended by CLSI（2008）.The genotype of TEM,SHV,CTX-M,IMP,VIM,OXA-23,OXA-24 and class I,II integrase was performed by polymerase chain reaction（PCR）.RESULTS Sensitive to antibiotics except Amikacin and carbapenem were low in 130 strains A.baumannii.The positive rates of 130 strains of TEM,SHV,CTX-M,Int1 and Int-2 were 68.5%,10.8%,17.7%,75.4% and 0,respectively.Positive rates of 51 strains carbapenem-resistant A.baumannii of IMP,VIM,OXA-23,OXA-24 were 25.5%,19.6%,72.5% and 0,respectively.CONCLUSION A.baumannii carries TEM,OXA-23,IMP,VIM and Int-2 type gene.Production of OXA-23 carbapenemase and metallo-β-lactamase in A.baumannii is the main mechanisms of carbapenem resistance in hospital.

关 键 词：鲍氏不动杆菌 Β-内酰胺酶 耐药性 

分 类 号：R378[医药卫生—病原生物学]