白细胞减少性鲍氏不动杆菌肺炎大鼠模型的构建  

Establishment of acinetobacter baumannii pneumonia model in rats with leucopenia

作　　者：阮世冲[1] 赵海金[1] 赖添顺[1] 蔡绍曦[1] 耿穂娜 

机构地区：[1]南方医科大学附属南方医院呼吸内科,广东广州510515 [2]南方医科大学附属南方医院检验科,广东广州510515

出　　处：《中华医院感染学杂志》2011年第10期1948-1951,共4页Chinese Journal of Nosocomiology

基　　金：广东省科技计划项目(2008A060202013)

摘　　要：目的构建白细胞减少性鲍氏不动杆菌肺炎的动物模型。方法 45只Sprague-Dawleg大鼠随机分为3组:空白组、白细胞减少组及模型组,各组15例;模型组接种细菌前4 d及接种前1 d分别腹腔注射环磷酰胺45 mg/kg和肌内注射地塞米松90 mg/kg,之后气管内滴注1.2×109CFU/ml鲍氏不动杆菌悬液;动态观察动物活动度、饮水及进食情况,同时观察肺组织湿干比(W/D)、病理变化并进行组间比较。结果模型组大鼠滴注鲍氏不动杆菌悬液后6 h,开始出现明显活动度、饮水及进食减少,同时湿干比增加W/D 5.21±0.52,对照组W/D3.00±0.44(P<0.05),病理切片见肺部中性粒细胞浸润、肺泡内出血等肺部炎症改变,36 h达到高峰然后开始改善,而其他各组干预后有轻度肺部炎症,但迅速恢复正常,接种鲍氏不动杆菌后,肺组织培养鉴定均可见鲍氏不动杆菌。结论该方法成功构建白细胞减少性鲍氏不动杆菌肺炎大鼠模型。OBJECTIVE To establish Acinetobacter baumannii pneumonia model in rats with leucopenia.METHODS Totally 45 Sprague-Dawleg rats were randomly divided into 3 groups：control group,immunosuppressive group and the model group（n=15）.The cyclophosphamide（45 mg/kg,intraperitoneal） and dexamethasone（90 mg/kg,intramuscular） were used 4d and 1d before A.baumannii inoculation in order to reduce the WBC counts in blood,and then innculated A.baumannii（1.2×109 CFU/ml,intratracheal） to establish pneumonia model.The activity,drinking and eating of the animals were observed dynamically.And the wet to dry ratio of weight of lung tissue and lung pathological change were monitored and compared among groups.RESULTS The activity,drinking and eating of the animals were significantly decreased and the wet to dry ratio was increased 6h after infusion of A.baumannii（5.21±0.52 vs 3.00±0.44,P0.05）.The pneumonia pathological change,such as neutrophil infiltration,alveolar hemorrhage appeared 6h after A.baumannii inoculation and it started to recover after 36h in the model group while light pneumonia change occurred and recovered soon after that in the other groups.A.baumannii was identified from samples of lung tissue.CONCLUSION A.baumannii pneumonia model has been successfully established in rats with leucopenia.

关 键 词：鲍氏不动杆菌 肺部炎症 白细胞减少症 免疫抑制 动物模型 

分 类 号：R378[医药卫生—病原生物学]