需氧厌氧瓶配对在血与体液病原菌培养中的临床价值  

Clinical value of aerobic and anaerobic culture bottles paired in pathogenic culture of blood and body fluid

作　　者：储从家[1] 吴惠玲[1] 孔繁林[1] 李杰芬[1] 管新龙[1] 杨宇溪[1] 孙丽[1] 

机构地区：[1]玉溪市人民医院检验科,云南玉溪653100

出　　处：《中华医院感染学杂志》2011年第10期2142-2144,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨需氧和厌氧培养方式的选择对不同种属病原菌检出率、检测时间的影响及常规使用的临床价值。方法使用mini VITAL或BacT/ALERT 3D血培养仪,及VITEK-32自动细菌鉴定仪对1999年1月-2009年12月38 839份血液与体液临床标本需氧和厌氧配对培养的检测结果作回顾性分析。结果 38 839份标本培养阳性7241例占18.64%,共分离出各种细菌56属171种7327株;在需氧和厌氧培养瓶中的阳性检出率差异有统计学意义(2χ=7.14,P<0.01),非发酵菌、微球菌属、真菌、厌氧菌阳性检出率差异有统计学意义(P<0.01),葡萄球菌属、棒杆菌属阳性检出率差异有统计学意义(P<0.05),沙门菌属和棒杆菌属细菌在需氧和厌氧培养两种瓶子的阳性检测速度差异有统计学意义(P<0.01)。结论采用需氧和厌氧瓶配对培养,既解决了培养结果的早期快速问题,又可提高阳性检出率,是一种理想的组合,在临床上有良好的应用价值。OBJECTIVE To study the clinical value on aerobic and anaerobic culture approaches and the effect on the recovery time and the recovery rate of different specific bacterial.METHODS Retrospective analysis of bacteria isolated from 38839 cases bottles of clinical blood and body fluid samples from Jan.1999 to Oct.2009 was performed by Mini VITAL Blood Culture Automaton System and VITEK32 Automicrobic System.RESULTS A total of the kind of 171 of 7327 strains of 56 isolates were recovered from 38839 blood samples and the result of 7241 was positive（18.64%）.The positive recovery rate of aerobic and anaerobic culture bottles of clinical separation aerobic of 7327 had significant difference（χ2=7.14,P0.01）,and the positive recovery rate of non-fermentation bacteria,Micro coccus,fungi and anaerobic bacteria had significant difference（P0.01）,and the positive recovery rate of staphylococcus and corynebacteria was significant（P0.05）.The positive recovery speed of Salmonella bacteria and corynebacteria bacteria isolates from aerobic and anaerobic culture bottles have significant difference（P0.01）.CONCLUSION The application both of aerobic and anaerobic culture is a reasonable suit,it is a kind of ideal combination,and it is good application value in clinic.

关 键 词：血液 体液 需氧培养 厌氧培养 配对 检出率 

分 类 号：R378[医药卫生—病原生物学]