骨关节结核患者外周血CD_4^+CD_(25)^+调节性T细胞的检测及意义  

CD_4^+CD_(25)^+ regulatory T cells in peripheral blood of patients with bone and joint tuberculosis:its detection and clinical significance

作　　者：张立群[1] 王云霞[1] 黄君富[1] 殷和[2] 张泽华[3] 王丹妮[1] 刘智勇[1] 夏涵[1] 府伟灵[1] 

机构地区：[1]第三军医大学第一附属医院检验科,重庆400038 [2]解放军第四医院检验科,青海西宁810007 [3]第三军医大学第一附属医院骨科,重庆400038

出　　处：《中华医院感染学杂志》2011年第10期2154-2156,共3页Chinese Journal of Nosocomiology

基　　金：国家传染病重大专项(2008ZX-10003-012;2008ZX10003-02);国家自然科学基金(81000776);重庆市自然科学基金(CSTC;2010BB5194);第三军医大学青年人才创新基金(2010XQN26)

摘　　要：目的通过对骨关节结核患者外周血CD4+CD25+调节性T细胞的分析,探讨其在骨关节结核患者外周血中表达及临床意义。方法采用流式细胞技术多色荧光分析方法,测定30例骨关节结核患者及30例健康志愿者外周血中CD4+CD25+调节性T细胞的表达水平,统计分析两组之间的差异。结果骨关节结核患者CD4+CD25+调节性T细胞所占比例为(13.62±3.15)%,与健康对照组(6.52±1.86)%相比差异有统计学意义(P<0.01);初治骨关节结核与复治骨关节结核差异有统计学意义(P<0.05)。结论 CD4+CD25+调节性T细胞可作为判断骨关节结核患者免疫功能的指标,它对骨关节结核患者的免疫状态、指导用药、疗效观察具有重要的临床意义。OBJECTIVE To explore the clinical significance by analyzeing CD4＋CD25＋ regulatory T cells（Treg） in the peripheral blood of patients with bone and joint tuberculosis.METHODS A series of 30 bone and joint tuberculosis patients were detected by flow cytometry with multicolor fluorescence technology for CD4＋CD25＋ regulator T cells,with 30 healthy persons as control group.The differences were analyzed between types of bone and joint tuberculosis,initial treatment and retreatment.RESULTS The percentage of CD4＋CD25＋ regulator T cells of bone and joint tuberculosis patients（13.62±3.15）% was significantly higher than that of controls（6.52±1.86）%（P0.01）.The difference between initial treatment and retreatment was significant（P0.05）.CONCLUSION CD4＋CD25＋ regulator T cells can be used as indicator of the immune function in bone and joint tuberculosis patients.It is of clinical significance for the observation of immune status,medication and curative effect on bone and joint tuberculosis patients.

关 键 词：骨关节结核 分枝杆菌 调节性T细胞 

分 类 号：R446[医药卫生—诊断学]