PKA介导的Aβ_(25-35)对钾通道(快速失活钾电流)的抑制  

Roles of PKA in Aβ_(25-35) induced inhibition of K^+ channels(fast inactivating K^+ current)

作　　者：姜慧霞[1,2] 谭爱娟[1] 张宇[1] 李建国[1] 高峰[1] 张鹏俊[3] 张策[1] 

机构地区：[1]山西医科大学生理系,太原030001 [2]山西医科大学物理教研室 [3]首都医科大学,北京100010

出　　处：《中国医疗前沿》2011年第6期50-51,共2页China Healthcare Innovation

摘　　要：目的观察PKA在Aβ25-35引起钾通道功能改变过程中的作用,阐明Aβ25-35引起钾通道功能异常的机制。方法酶解急性分离大鼠神经元;利用全细胞膜片钳技术记录电压依赖型钾电流。结果 PKA激动剂8-brom-camp抑制IA电流;PKA阻断剂H-89拮抗Aβ25-35诱导的对IA电流的抑制。结论 PKA参与了Aβ25-35对大鼠海马CA1区神经元电压依赖性钾电流的抑制。Objective To investigate the mechanism of PKA in Aβ25-35 induced K＋ channel dysfunction.Methods After rats hippocampal CA1 neurons were isolated by protease,voltage-dependent K＋ current were recorded using whole cell patch clamp technique.Results PKA agonist inhibited IA currents;PKA inhibitor antagonized Aβ25-35 induced inhibition of IA currents.Conclusion PKA involved in Aβ25-35 inhibition of voltage-dependent K＋ current on rat hippocampal CA1 neurons.

关 键 词：瞬时外向钾电流(IA) β淀粉样蛋白(Aβ) PKA激动剂 PKA抑制剂 

分 类 号：R338.2[医药卫生—人体生理学] R749.1[医药卫生—基础医学]