头孢西丁纸片法检测MRSA的评价  

The Evaluate of the Method of Cefoxitin for Delection Methicillin-Resistant Staphylococcus aureus(MRSA)

作　　者：王燕[1] 范琳[1] 彭慧平[1] 

机构地区：[1]大连大学附属新华医院检验科,116012

出　　处：《中国医疗前沿》2011年第6期78-78,79,共2页China Healthcare Innovation

摘　　要：目的评价头孢西丁纸片法检测耐甲氧西林金黄色葡萄球菌(MRSA)的方法。方法采用头孢西丁纸片法(30μg/片)和苯唑西林纸片法(1.0μg/片)检测MRSA,以苯唑西林琼脂平板筛选法为参考方法。结果用苯唑西林琼脂平板筛选法检测的98株金黄色葡萄球菌,其中MRSA有72株,用头孢西丁纸片法筛选MRSA敏感性为100%,特异性为100%,苯唑西林纸片法检测MRSA敏感性为94.4%,特异性为96.2%。结论头孢西丁纸片法与苯唑西林琼脂平板筛选法一样,是检测MRSA的可靠方法。Objective To establish and evaluate the method for detection of Methicillin-Resistant Staphylococcus aureus（MRSA）.Methods The disks with 30 microgramme of cefoxitin and 1 microgramme of oxcillin were used for the detection of MRSA with oxacillin agar dilution test as the reference.Results Of the 98 test isolates of Staphylococcus aureus,72 isolates showed the MRSA by oxacillin agar dilution test.In cefoxitin disk diffusion tests,the sensitivity was 100%,and the specificity was 100%.In oxacillin diffusion tests,the sensitivity was 94.4%,and the specificity was 96.2%.Conclusion Cefoxitin disk diffusion method can be used reliably in detecting and confirming MRSA.

关 键 词：耐甲氧西林金黄色葡萄球菌(MRSA) 头孢西丁 纸片扩散法 

分 类 号：R446.5[医药卫生—诊断学]