PRK基因原核表达质粒的构建  

Construction of PRK Gene Plasmid Expressing in Prokaryotic System

作　　者：龚元亚[1] 代其林[1] 田霞[1] 孙英坤[1] 谢琳[1] 杨娟[1] 王劲[1,2] 

机构地区：[1]西南科技大学植物生物技术研究中心,四川绵阳621010 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《南方农业》2011年第3期13-15,22,共4页South China Agriculture

基　　金：转基因生物新品种培育重大专项(2009ZX08009-091B);国家自然科学基金(30871555);教育部新世纪优秀人才支持计划(NCET-08-0940);四川省教育厅科技项目(09ZA034)

摘　　要：以拟南芥cDNA为模板,扩增出PRK基因,构建重组质粒pET-28a(+)-PRK转化大肠杆菌DH5α。经PCR鉴定和酶切鉴定筛选出阳性克隆,将阳性克隆的质粒转化到大肠杆菌表达载体BL21中,构建PRK基因原核表达载体。测序结果表明pET-28a(+)-PRK载体构建成功。The cDNA extracted from Arabidopsis thaliana are employed as a template,PRK gene fragment is amplified by PCR.The construction of recombinant plasmid pET28a（＋）-PRK is transferred into E.coli DH5α,the positive clones are screened by PCR and enzyme digestion,which are transferred into E.coli BL21.Construction of PRK gene plasmid expressing.The sequencing result shows that pET-28a（＋）-PRK carrier construction are built successfully.

关 键 词：PRK基因 载体构建 PCR 

分 类 号：Q786[生物学—分子生物学]