新的M家族芋螺毒素的克隆及进化分析  

Cloning and evolution analysis of novel M-family conotoxins

作　　者：孙婷[1,2] 刘珠果[2] 吴传侠[2] 赵玉娥[1] 戴秋云[2] 

机构地区：[1]北京联合大学生物化学工程学院,北京100023 [2]军事医学科学院生物工程研究所,北京100071

出　　处：《军事医学》2011年第4期249-252,共4页Military Medical Sciences

基　　金：国家973计划项目(2010CB529802);国家高技术研究发展计划(863计划)(2006AA09Z404);国家自然科学基金项目(9071028)

摘　　要：目的从中国南海芋螺中寻找新的M家族芋螺毒素。方法应用3′RACE及巢式PCR进行基因克隆,将得到的目的基因连接入pGM-T载体、转化并测序,用进化分析软件对得到的新序列进行进化分析。结果从6种芋螺毒腺管中获得8个新的M家族芋螺毒素前体肽序列,其成熟肽含有15~21个氨基酸残基。结论这些新的M族毒素与已报道的M家族毒素序列同源性较低,是Mini-M家族的新成员。Objective To identify new M-family conotoxins from six Conus species in the South China Sea.Methods Conotoxin genes were cloned by 3′RACE and nested PCR and ligated into the pGM-T vector and transformed into DH5α competent cells.The positive clones were sequenced.The evolutionary tree was analyzed using software ClustalX1.83 and Mega4.0.Results Eight novel M-family conotoxin precursors were cloned from six Conus species from the South China Sea.The mature peptides contained 15-21 amino acid residues.Conclusion The novel M-family conotoxins exhibit less homology with the reported M-family conotoxins,and are new members of Mini-M conotoxins.

关 键 词：芋螺毒素类 M家族 克隆 进化 

分 类 号：R996.3[医药卫生—毒理学]