斑鳜轻链3基因(MLC3)cDNA的克隆及纵向表达分析  

cDNA Cloning and Vertical Expression Analysis of the Myosin Light Chain 3(MLC3)Gene in Siniperca Scherzeri

作　　者：农小献[1,2] 赵发兰[1,2] 宾石玉[1] 陈敦学[1,2] 刘希良[1,2] 史竸[1,2] 褚武英[2] 

机构地区：[1]广西师范大学生命科学学院,中国桂林541004 [2]长沙学院生物工程与环境科学系,中国长沙410003

出　　处：《湖南师范大学自然科学学报》2011年第2期79-83,共5页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金资助项目(30972263);湖南省教育厅重点资助项目(09A008);湖南省高校科技创新团队资助项目(湘教通[2008]49号)

摘　　要：提取斑鳜肌肉组织总RNA,逆转录得到cDNA,然后利用常规PCR法扩增得到斑鳜肌球蛋白轻链3基因(MLC3)序列.斑鳜MLC3基因cDNA序列的全长为532 bp,编码150个氨基酸.通过PROSITE tools软件预测显示该序列具有2个EF-手相结构.通过实时荧光定量PCR法对斑鳜MLC3纵向表达进行分析发现,MLC3在斑鳜背肌的近头部、中部和近尾部都有表达,近头部和中部的表达量差异不显著,近尾部表达量显著高于近头部和中部.RNA of Siniperca scherzeri was isolated from muscle tissue, applied differential display reversed transcripted PCR （DDRT-PCR） technique to get cDNA sequence of MLC3, which is 532 bp in length, and en- codes a 150 amino acid peptide. It is found that it contains two EF-hand motifs by using the indicating PROSITE tools software. Longitudinal expression of mandarin fish was studied by using Real-time Quantitative PCR showed that the MLC3 mRNA was expressed along longitudinal axis with unsignificant between the anterior and middle muscle positions, but significant variation between the posterior and anterior, middle muscle positions.

关 键 词：斑鳜 肌球蛋白轻链3(MLC3) 纵向表达 

分 类 号：Q781[生物学—分子生物学] Q785